SPI-112

Catalog No.S0244 Batch:S024401

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Technical Data

Formula

C22H17FN4O5S

Molecular Weight 468.46 CAS No. 1051387-90-6
Solubility (25°C)* In vitro DMSO 94 mg/mL (200.65 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description SPI-112 is a not cell permeable inhibitor of Src homology region 2 domain-containing phosphatase 2 (SHP-2, PTPN11) with IC50 of 1 μM in cell-free assay.
Targets
SHP-2 [1]
(Cell-free assay)
1 μM
In vitro

SPI-112 is a not cell permeable inhibitor of Src homology region 2 domain-containing phosphatase 2 (SHP-2, PTPN11) with IC50 of 1.0 μM, suggesting that SPI-112 binds to the catalytic pocket.[1]

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    MDA-MB-468 breast cancer cells

  • Concentrations

    100 μM

  • Incubation Time

    10 min

  • Method

    Sub-confluent MDA-MB-468 cells were serum-starved in DMEM/0.1% BSA for 18 h, pretreated overnight with SPI-112 (100 μM) and stimulated with EGF (50 ng/ml, 10 min) or mocked treated as indicated. Shp2 was immunoprecipitated from cell lysate supernatants and Shp2 PTP activity was measured.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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