Sodium Tauroursodeoxycholate (TUDC)

Catalog No.S7896 Batch:S789601

Technical Data

Formula	C₂₆H₄₄NNaO₆S
Molecular Weight	521.69	CAS No.	35807-85-3
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (191.68 mM)
		Water	100 mg/mL (191.68 mM)
		Ethanol	71 mg/mL (136.09 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Sodium Tauroursodeoxycholate (TUDC) is a water soluble bile salt, used for the treatment of gallstones and liver cirrhosis.
In vitro	In vitro, Tauroursodeoxycholate shows protective and reversing effects on 17βEG-induced impairment of the cVA-of-CLF. ^[1] In Mz-ChA-1 cholangiocarcinoma cells, Tauroursodeoxycholate inhibits cell growth via Ca²⁺-, PKC-, and MAPK-dependent pathways. ^[3] In isolated rat hepatocytes, Tauroursodeoxycholate inhibits bile acid-Induced Apoptosis via a β1-integrin-mediated formation of cAMP. ^[4]
In vivo	In phalloidin-induced cholestasis rats, Tauroursodeoxycholate (360 μmol/kg, i.v.) significantly suppresses the decrease in bile flow and increases in serum alkaline phosphatase, leucine aminopeptidase and glutamic pyruvic transaminase activities, cholesterol, phospholipid and bile acid concentrations. ^[2]

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines Mz-ChA-1 cells Concentrations ~200 μM Incubation Time 96 h Method After trypsinization, Mz-ChA-1 cells are suspended in CCM at 5 × 10⁴ cells/mL, and 200 μL of the cell suspension are seeded into flat-bottomed 96-well plates. After an initial incubation step of 4 h at 37°C in a 5% CO² incubator, the cells are incubated at 37°C with TUDCA (0.2–200 μM). After incubation for 24–96 h, [³H]thymidine is put into each well at 5.0 μCi/mL and incubated for additional 4 h. [³H]thymidine incorporation is measured by a scintillation counter. At the end of each incubation period, cell viability is evaluated by trypan blue exclusion.
Animal Study:^{^[2]}	Animal Models Phalloidin-induced cholestasis rats Dosages 360 μmol/kg Administration i.v.

Cell Assay:^{^[3]}

Cell lines
Mz-ChA-1 cells
Concentrations
~200 μM
Incubation Time
96 h
Method
After trypsinization, Mz-ChA-1 cells are suspended in CCM at 5 × 10⁴ cells/mL, and 200 μL of the cell suspension are seeded into flat-bottomed 96-well plates. After an initial incubation step of 4 h at 37°C in a 5% CO² incubator, the cells are incubated at 37°C with TUDCA (0.2–200 μM). After incubation for 24–96 h, [³H]thymidine is put into each well at 5.0 μCi/mL and incubated for additional 4 h. [³H]thymidine incorporation is measured by a scintillation counter. At the end of each incubation period, cell viability is evaluated by trypan blue exclusion.

Animal Study:^{^[2]}

Animal Models
Phalloidin-induced cholestasis rats
Dosages
360 μmol/kg
Administration
i.v.

References

[4] Sommerfeld A, et al. Cell Physiol Biochem. 2015, 36(3), 866-883.

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Selleck's Sodium Tauroursodeoxycholate (TUDC) has been cited by 5 publications

Cetylpyridinium chloride triggers paraptosis to suppress pancreatic tumor growth via the ERN1-MAP3K5-p38 pathway [ iScience, 2024, 27(8):110598]	PubMed: 39211547
ER-transiting bacterial toxins amplify STING innate immune responses and elicit ER stress [ Infect Immun, 2024, 92(8):e0030024]	PubMed: 39057915
Antibiotic-modulated microbiome suppresses lethal inflammation and prolongs lifespan in Treg-deficient mice. [ Microbiome, 2019, 7(1):145]	PubMed: 31699146
Melatonin Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib through the PERK-ATF4-Beclin1 Pathway. [ Int J Biol Sci, 2019, 15(9):1905-1920]	PubMed: 31523192
Endoplasmic reticulum stress is activated in post-ischemic kidneys to promote chronic kidney disease. [ EBioMedicine, 2018, 37:269-280]	PubMed: 30314894

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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