SN-011

Catalog No.E1066 Batch:E106603

Technical Data

Formula	C₂₅H₁₉FN₂O₄S
Molecular Weight	462.49	CAS No.	2249435-90-1
Solubility (25°C)*	In vitro	DMSO	93 mg/mL (201.08 mM)
		Ethanol	6 mg/mL (12.97 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

SN-011 is a STING-specific antagonist with IC50 of 76 nM.

Targets

STING ^[1]

76 nM

In vitro

SN-011 substantially suppresses 2′3′-cGAMP-induced STING oligomerization and phosphorylation assessed by immunoblot in HFFs. SN-011 specifically inhibits STING activation and STING-dependent signaling.^[1]

In vivo

Serum antinuclear antibody is markedly reduced by SN-011 treatment. SN-011 also significantly reduces the number of activated CD69⁺ CD8 T cells and memory CD44^high CD62L^low CD4 and CD8 T cells to near normal levels in the spleens of treated Trex1^−/− mice. SN-011 strongly reduces inflammation and protects Trex1^−/− mice from death.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HFFs Concentrations 1 μM - 10 μM Incubation Time 24 h Method HFFs are harvested and lysed in lysis buffer with brief sonication. Cell lysate is collected and incubated with biotin (5 μM). For purified recombinant STING-CTD protein, 0.05 mg protein in 400 μL buffer (25 mM Tris-HCl, pH 7.5, 150 mM NaCl) was incubated with biotin (5 μM) for 1 h at 4 °C. Then the protein of interest was pull-down by streptavidinconjugated agarose and detected by immunoblot analysis.
Animal Study:^{^[1]}	Animal Models Trex1^−/− mice Dosages 5 mg/kg, 10 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
HFFs
Concentrations
1 μM - 10 μM
Incubation Time
24 h
Method
HFFs are harvested and lysed in lysis buffer with brief sonication. Cell lysate is collected and incubated with biotin (5 μM). For purified recombinant STING-CTD protein, 0.05 mg protein in 400 μL buffer (25 mM Tris-HCl, pH 7.5, 150 mM NaCl) was incubated with biotin (5 μM) for 1 h at 4 °C. Then the protein of interest was pull-down by streptavidinconjugated agarose and detected by immunoblot analysis.

Animal Study:^{^[1]}

Animal Models
Trex1^−/− mice
Dosages
5 mg/kg, 10 mg/kg
Administration
i.p.

References

[1] Ze Hong, et al. Proc Natl Acad Sci U S A . 2021 Jun 15;118(24):e2105465118.

Selleck's SN-011 has been cited by 1 publication

YTHDF2 in peritumoral hepatocytes mediates chemotherapy-induced antitumor immune responses through CX3CL1-mediated CD8+ T cell recruitment [ Mol Cancer, 2024, 23(1):186]	PubMed: 39237909

YTHDF2 in peritumoral hepatocytes mediates chemotherapy-induced antitumor immune responses through CX3CL1-mediated CD8+ T cell recruitment [ Mol Cancer, 2024, 23(1):186]

PubMed: 39237909

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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