SMER3

Catalog No.S4925 Batch:S492502

Technical Data

Formula

C₁₁H₄N₄O₂

Molecular Weight

224.18

CAS No.

67200-34-4

Solubility (25°C)*

In vitro

DMSO

15 mg/mL (66.91 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	0.15mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 3 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.55mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 11 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	SMER3, the small-molecule enhancer of rapamycin (SMER), is an inhibitor of the Skp1-Cullin-F-box (SCF)^Met30 ubiquitin ligase with IC50 of 51 nM.
In vitro	SMER3 specifically inhibits ubiquitination by recombinant reconstituted SCF^Met30, selectively disassembles or prevents assembly of SCF^Met30 but not other SCF complexes, and directly binds to Met30 (or Met30-Skp1 complex), but not Skp1 alone, which inhibits Met30 bounding to Skp1. SMER3 treatment is able to upregulate a set of methionine biosynthesis genes. SMER3 enhances rapamycin’s growth inhibitory effect by inhibition of SCF^Met30 and blocking Met4 ubiquitination. ^[1]

Description

SMER3, the small-molecule enhancer of rapamycin (SMER), is an inhibitor of the Skp1-Cullin-F-box (SCF)^Met30 ubiquitin ligase with IC50 of 51 nM.

In vitro

SMER3 specifically inhibits ubiquitination by recombinant reconstituted SCF^Met30, selectively disassembles or prevents assembly of SCF^Met30 but not other SCF complexes, and directly binds to Met30 (or Met30-Skp1 complex), but not Skp1 alone, which inhibits Met30 bounding to Skp1. SMER3 treatment is able to upregulate a set of methionine biosynthesis genes. SMER3 enhances rapamycin’s growth inhibitory effect by inhibition of SCF^Met30 and blocking Met4 ubiquitination. ^[1]

Protocol (from reference)

References

[1] Aghajan M, et al. Nat Biotechnol. 2010, 28(7), 738-742.

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.