SMER3

Catalog No.S4925 Batch:S492501

Technical Data

Formula	C₁₁H₄N₄O₂
Molecular Weight	224.18	CAS No.	67200-34-4
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description	SMER3, the small-molecule enhancer of rapamycin (SMER), is an inhibitor of the Skp1-Cullin-F-box (SCF)^Met30 ubiquitin ligase with IC50 of 51 nM.
In vitro	SMER3 specifically inhibits ubiquitination by recombinant reconstituted SCF^Met30, selectively disassembles or prevents assembly of SCF^Met30 but not other SCF complexes, and directly binds to Met30 (or Met30-Skp1 complex), but not Skp1 alone, which inhibits Met30 bounding to Skp1. SMER3 treatment is able to upregulate a set of methionine biosynthesis genes. SMER3 enhances rapamycin’s growth inhibitory effect by inhibition of SCF^Met30 and blocking Met4 ubiquitination. ^[1]

Description

SMER3, the small-molecule enhancer of rapamycin (SMER), is an inhibitor of the Skp1-Cullin-F-box (SCF)^Met30 ubiquitin ligase with IC50 of 51 nM.

In vitro

SMER3 specifically inhibits ubiquitination by recombinant reconstituted SCF^Met30, selectively disassembles or prevents assembly of SCF^Met30 but not other SCF complexes, and directly binds to Met30 (or Met30-Skp1 complex), but not Skp1 alone, which inhibits Met30 bounding to Skp1. SMER3 treatment is able to upregulate a set of methionine biosynthesis genes. SMER3 enhances rapamycin’s growth inhibitory effect by inhibition of SCF^Met30 and blocking Met4 ubiquitination. ^[1]

Protocol (from reference)

References

[1] Aghajan M, et al. Nat Biotechnol. 2010, 28(7), 738-742.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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