SF1670

Catalog No.S7310 Batch:S731001

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Technical Data

Formula

C19H17NO3

Molecular Weight 307.34 CAS No. 345630-40-2
Solubility (25°C)* In vitro DMSO 29 mg/mL (94.35 mM)
Ethanol 1 mg/mL (3.25 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
0.13mg/ml Taking the 1 mL working solution as an example, add 50 μL 2.5 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description SF1670 is a highly potent and specific PTEN inhibitor with IC50 of 2 μM.
Targets
PTEN [1]
2 μM
In vitro SF1670 shows potent cytotoxicity in HBEC, PC-3, H1299 cells with IC50 of 5 μM, 10 μM, 44 μM, respectively. In a mouse aortic ring matrigel angiogenesis model, SF1670 stimulates the angiogenic processes. [1] SF1670 augments chemoattractant-elicited PtdIns(3,4,5)P3 signaling in neutrophils and enhances neutrophil functions. [2]
In vivo Pretreatment of SF1670 (500 nM i.v.) augments bacteria-killing capability in neutropenic mice in both peritonitis and bacterial pneumonia, and decreases the mortality of neutropenia-related pneumonia. [2]

Protocol (from reference)

Kinase Assay:[1]
  • PTEN Inhibition Assay

    To determine the dose response of potential PTEN inhibitors, doses of test compounds ranging from 1 nM to 250 uM (final reaction mix concentrations) are evaluated in the general PTEN inhibition assay. To obtain performed IC50 data, two separate rounds of the dose response assay are performed. In the first round, PTEN activity is tested in the presence of inhibitor at 10 fold serial dilutions ranging from 1 nM to 250 uM. Once the concentration range is determined, at which PTEN activity changes dramatically, two additional concentration data points within this range are added and the PTEN inhibition assay is then rerun for the second round. The PTEN inhibition IC50 is presented as the inhibitor concentration at which 50% of the PTEN activity. When the assay was run on multiple occasions and gave slightly different IC50 then those are reported as a range of IC50 found.

Cell Assay:[1]
  • Cell lines

    Human brain endothelial cells (HBEC), human prostate cancer cells (PC-3) and human non-small cell lung cancer cells (H1299)

  • Concentrations

    ~1 mM

  • Incubation Time

    2 hours

  • Method

    Cells are plated into 96-well plates in RPMI 1640 medium supplemented with 10% FBS and incubated overnight at 37°C. in an incubator containing an atmosphere of 5% CO2. The following day, the medium is replaced and cells are starved by placing in 100 uL of serum-free medium for 3 hours. Serially diluted test compounds are added to the wells and incubated with the cells for 2 hours at 37°C. Compounds are tested in a range from 1 mM to 0.1 nM depending on solubility. MTT is added to the wells at a final concentration of 5 μg/ml and incubated with the cells for 3 more hours. At the end of the incubation, the medium is aspirated and the MTT stain in the cells is dissolved by the addition of 100 μL DMSO. Optical density of each well is then measured at 570 nm using a SpectroMax Plus spectrophotometric plate reader. The IC50 determination from the data was made using Prism software.

Animal Study:[2]
  • Animal Models

    Neutropenic mice

  • Dosages

    500 nM

  • Administration

    Tail-vein injection

Customer Product Validation

Data from [Data independently produced by , , Int J Oncol, 2017, 51(3):899-906]

Data from [Data independently produced by , , Biochem Biophys Res Commun, 2017, 483(1):712-717]

Selleck's SF1670 has been cited by 25 publications

African swine fever virus infection regulates pyroptosis by cleaving gasdermin A (GSDMA) via active caspase-3 and caspase-4 [ J Biol Chem, 2024, S0021-9258(24)01808-8] PubMed: 38657868
TAN (tannic acid) inhibits BPA-induced pyroptosis of L8824 (grass carp hepatocytes) by regulating PTEN/PI3K/AKT pathway [ Fish Shellfish Immunol, 2024, 146:109384] PubMed: 38246267
Nicotine Decreases Nerve Regeneration and Pain Behaviors via PTEN and Downstream Inflammation-Related Pathway in Two Rat Nerve Injury Models [ eNeuro, 2023, 10(9)ENEURO.0185-23.2023] PubMed: 37620149
Nicotine Decreases Nerve Regeneration and Pain Behaviors via PTEN and Downstream Inflammation-Related Pathway in Two Rat Nerve Injury Models [ eNeuro, 2023, 10(9)ENEURO.0185-23.2023] PubMed: 37620149
PTEN/AKT signaling pathway related to hTERT downregulation and telomere shortening induced in Toxoplasma GRA16-expressing colorectal cancer cells [ Biomed Pharmacother, 2022, 153:113366] PubMed: 35810694
Formononetin inhibits IL-1β-induced inflammation in human chondrocytes and slows the progression of osteoarthritis in rat model via the regulation of PTEN/AKT/NF-κB pathway [ Int Immunopharmacol, 2022, 113(Pt A):109309] PubMed: 36306560
Exosomal miR-10b-5p mediates cell communication of gastric cancer cells and fibroblasts and facilitates cell proliferation [ J Cancer, 2021, 12(7):2140-2150] PubMed: 33754012
Assessment of Alveolar Macrophage Dysfunction Using an in vitro Model of Acute Respiratory Distress Syndrome [ Front Med (Lausanne), 2021, 8:737859] PubMed: 34660643
Three paralogous clusters of the miR-17~92 family of microRNAs restrain IL-12-mediated immune defense. [ Cell Mol Immunol, 2020, 10.1038/s41423-020-0363-5] PubMed: 32015501
MicroRNAs of the miR-17~9 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression [ Elife, 2020, 9e55676] PubMed: 33150865

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.