Sesamol

Catalog No.S3626 Batch:S362601

Technical Data

Formula	C₇H₆O₃
Molecular Weight	138.12	CAS No.	533-31-3
Solubility (25°C)*	In vitro	DMSO	27 mg/mL (195.48 mM)
		Water	27 mg/mL (195.48 mM)
		Ethanol	27 mg/mL (195.48 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Sesamol (1,3-Benzodioxol-5-ol, 3,4-Methylenedioxyphenol), a natural organic compound, is regarded as a major antioxidant component in the oil with chemoprevention, antimutagenic, and antihepatotoxic activities. It induces apoptosis of cancer and cardiovascular cells.
In vitro	Sesamol treatment suppresses colony formation, elicits S phase arrest during cell cycle progression, and induces both intrinsic and extrinsic apoptotic pathway in vitro with a dose-dependent manner. Furthermore, sesamol treatment elicits mitochondrial dysfunction by inducing a loss of mitochondrial membrane potential. Impaired mitochondria and accumulated H2O2 production results in disturbance of redox-sensitive signaling including Akt and MAPKs pathways. Mitochondrial biogenesis is inhibited as suggested by the decline in expression of mitochondrial complex I subunit ND1, and the upstream AMPK/PGC1α signals. Importantly, sesamol inhibits mitophagy and autophagy through impeding the PI3K Class III/Belin-1 pathway. Autophagy stimulator rapamycin reverses sesamol-induced apoptosis and mitochondrial respiration disorders^[1]. Sesamol increases the activity of caspase 8, 9, and 3/7, indicating that apoptotic cell death occurred through both extrinsic and intrinsic pathways. Sesamol causes the loss of mitochondrial transmembrane potential signifying intrinsic apoptosis induction in human lung adenocarcinoma (SK-LU-1) cells^[2].
In vivo	Sesamol has potent anti-hepatoma activity in a xenograft nude mice model^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HepG2 cells, BRL-3A cells Concentrations 0, 0.01, 0.05, 0.1, 0.2, 0.5 or 1 mM Incubation Time 24 h Method The cytotoxicity of sesamol on HepG2 cells and BRL-3A cells is examined by using MTT assay. Briefly, cells in exponential growth are seeded into 96-well plates at a density of 1 × 10⁵ cells/mL. The cells are then treated with sesamol at various doses (0, 0.01, 0.05, 0.1, 0.2, 0.5 or 1 mM). After 24 h, the supernatant is discarded and 100 μL FBS free medium is added. 100 μL of MTT is added in each well, and cells are further incubated for 4 h in the dark. After incubation, the medium is discarded, 100 μL of DMSO is added, and the optical density of each well is measured at 570 nm.
Animal Study:^{^[1]}	Animal Models Balb/c nu/nu mice Dosages 100 mg/kg or 200 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
HepG2 cells, BRL-3A cells
Concentrations
0, 0.01, 0.05, 0.1, 0.2, 0.5 or 1 mM
Incubation Time
24 h
Method
The cytotoxicity of sesamol on HepG2 cells and BRL-3A cells is examined by using MTT assay. Briefly, cells in exponential growth are seeded into 96-well plates at a density of 1 × 10⁵ cells/mL. The cells are then treated with sesamol at various doses (0, 0.01, 0.05, 0.1, 0.2, 0.5 or 1 mM). After 24 h, the supernatant is discarded and 100 μL FBS free medium is added. 100 μL of MTT is added in each well, and cells are further incubated for 4 h in the dark. After incubation, the medium is discarded, 100 μL of DMSO is added, and the optical density of each well is measured at 570 nm.

Animal Study:^{^[1]}

Animal Models
Balb/c nu/nu mice
Dosages
100 mg/kg or 200 mg/kg
Administration
i.p.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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