Sephin-1

Catalog No.S6391 Batch:S639101

Technical Data

Formula	C₈H₉ClN₄
Molecular Weight	196.64	CAS No.	951441-04-6
Solubility (25°C)*	In vitro	DMSO	39 mg/mL (198.33 mM)
		Ethanol	39 mg/mL (198.33 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Sephin-1 (NSC-65390) is a selective inhibitor of a holophosphatase, a small molecule that safely and selectively inhibits a regulatory subunit of protein phosphatase 1 in vivo. Sephin-1 (NSC-65390) selectively bounds and inhibits the stress-induced PPP1R15A.

Targets

holophosphatase ^[1]

In vitro

Sephin1 stimulates ATF4 synthesis in the absence of stress.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HEK293 and HeLa cells Concentrations 10 µM Incubation Time 1.5, 3, 6, 18 to 24 h Method To characterize UPR activation, HEK293 cells and primary cortical neurons were treated with Thap (100 nM) alone or in combination with the UPR modulators described above [ISRIB (500 nM), GSK (2 µM), or 4µ8C (5, 16, and 50 µM), and Sephin1 (10 µM), and DMSO as a control]. Cells were harvested for Western blot (WB) analysis at different time points after treatment (from 1.5, 3, 6, 18 to 24 h).

Cell Assay:^{^[2]}

Cell lines
HEK293 and HeLa cells
Concentrations
10 µM
Incubation Time
1.5, 3, 6, 18 to 24 h
Method
To characterize UPR activation, HEK293 cells and primary cortical neurons were treated with Thap (100 nM) alone or in combination with the UPR modulators described above [ISRIB (500 nM), GSK (2 µM), or 4µ8C (5, 16, and 50 µM), and Sephin1 (10 µM), and DMSO as a control]. Cells were harvested for Western blot (WB) analysis at different time points after treatment (from 1.5, 3, 6, 18 to 24 h).

References

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SHIPPING AND STORAGE
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