Secukinumab (anti-IL-17A)

Catalog No.A2025        Batch: A202501

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Technical Data

CAS No. 1229022-83-6
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source Human
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99.82%
Protein concentration 5.00mg/ml
Endotoxin Level <1EU/mg

Biological Activity

Description Secukinumab (anti-IL-17A) (AIN457) is a human IgG1κ monoclonal antibody that binds to the protein interleukin (IL)-17A, and is applied for the treatment of psoriasis, ankylosing spondylitis, and psoriatic arthritis. MW=147.9 kDa.
In vitro

Secukinumab can reduce cell growth in the miR130b-knockdown OCI-ly10 co-culture system.[1]
In vivo

Secukinumab administration significantly attenuates AAV8-IRF8 mediated recruitment of neutrophils, also downregulates the autophagy level and inhibits the release of either autophagy-dependent or independent chemokines in vivo.[2]

Protocol (Only for Reference)

Cell Assay:

  • MTT assay was adopted to measure cell growth in the control inhibitor or miR130b inhibitor transfected OCI-ly10 co-culture system upon treatment with or without IL17 inhibitor.
Animal Study:

  • Animal Models: C57BL/6 mice, IRF8 FLOX mice (C57BL/6J background)
    Dosages: 200 μg
    Administration: i.p.
    Reference: https://pubmed.ncbi.nlm.nih.gov/34400126/
     

Selleck's Secukinumab (anti-IL-17A) has been cited by 2 publications

Overexpression of microRNA-145 enhanced docetaxel sensitivity in breast cancer cells via inactivation of protein kinase B gamma-mediated phosphoinositide 3-kinase -protein kinase B pathway [ Signal Transduct Target Ther, 2022, 7(1):80] PubMed: 35301282
Hepatic interferon regulatory factor 8 expression mediates liver ischemia/reperfusion injury in mice [ Biochem Pharmacol, 2021, 192:114728] PubMed: 34400126

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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