Scutellarin

Catalog No.S3810 Batch:S381003

Technical Data

Formula	C₂₁H₁₈O₁₂
Molecular Weight	462.36	CAS No.	27740-01-8
Solubility (25°C)*	In vitro	DMSO	92 mg/mL (198.97 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Scutellarin (Breviscapine, Breviscapin, Scutellarein-7-glucuronide), the major active principal flavonoids extracted from the Chinese herbal medicines Scutellaria baicalensis and Erigeron breviscapus (Vant.) Hand-Mazz, has many pharmacological effects, such as antioxidant, antitumor, antiviral, and antiinflammatory activities. Scutellarin can down-regulates the STAT3/Girdin/Akt signaling in HCC cells, and inhibits RANKL-mediated MAPK and NF-κB signaling pathway in osteoclasts.

Targets

STAT3 ^[6]

Akt ^[6]

NF-κB ^[7]

In vitro

Scutellarin protects PC12 cells against H2O2-induced cytotoxicity. It attenuates H2O2-induced intracellular accumulation of ROS and lipid peroxidation^[3]. Scutellarin shows negligible inhibitory effects on the six major CYP isoenzymes in human/rat liver microsomes with almost all of the IC50 values exceeding 100 μM, whereas it shows values of 63.8 μM for CYP2C19 in human liver microsomes, and 63.1 and 85.6 μM for CYP2C7 and CYP2C79 in rat liver microsomes, respectively. Scutellarin also has weak inhibitory effect on P-gp^[4]. Scutellarin strongly induces MMP-2 activation and mRNA expression in cultured HUVECs in a concentration-dependent manner. It promotes angiogenesis and may form a basis for angiogenic therapy^[5].

In vivo

The pharmacokinetic results indicate that scutellarin undergoes rapid and extensive biotransformation in vivo. After intraperitoneal injection. scutellarin is absorbed rapidly. The relative bioavailability of oral administration is very low (10.67 %±4.78 %)^[1]. Scutellarin has a series of protective effects including dilate blood vessels, improving microcirculation, decreasing the viscosity of blood, reducing the blood platelet count, inhibiting platelet aggregation activity etc^[2].

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines PC12 cells Concentrations 1 and 10 μmol/L Incubation Time 30 min Method Experiments are carried out 24-48 h after the cells are seeded into plates or dishes. To produce an oxidative stress, H2O2 is freshly prepared from a 30% stock solution prior to each experiment. Preincubation of cells with scutellarin is conducted for 30 min before H2O2 is added. Assays for cell viability, ROS, lipid peroxide, and apoptosis are performed at 6 or 12 h after addition of H2O2.
Animal Study:^{^[2]}	Animal Models Male Wistar rats Dosages 0.09, 0.17, 0.35, 0.70, 1.40 mmol/kg Administration oral

Cell Assay:^{^[3]}

Cell lines
PC12 cells
Concentrations
1 and 10 μmol/L
Incubation Time
30 min
Method
Experiments are carried out 24-48 h after the cells are seeded into plates or dishes. To produce an oxidative stress, H2O2 is freshly prepared from a 30% stock solution prior to each experiment. Preincubation of cells with scutellarin is conducted for 30 min before H2O2 is added. Assays for cell viability, ROS, lipid peroxide, and apoptosis are performed at 6 or 12 h after addition of H2O2.

Animal Study:^{^[2]}

Animal Models
Male Wistar rats
Dosages
0.09, 0.17, 0.35, 0.70, 1.40 mmol/kg
Administration
oral

References

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Selleck's Scutellarin has been cited by 1 publication

Scutellarin ameliorates osteoarthritis by protecting chondrocytes and subchondral bone microstructure by inactivating NF-κB/MAPK signal transduction [ Biomed Pharmacother, 2022, 155:113781]	PubMed: 36271561

Scutellarin ameliorates osteoarthritis by protecting chondrocytes and subchondral bone microstructure by inactivating NF-κB/MAPK signal transduction [ Biomed Pharmacother, 2022, 155:113781]

PubMed: 36271561

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SHIPPING AND STORAGE
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