Scutellarin

Catalog No.S3810 Batch:S381002

Technical Data

Formula

C₂₁H₁₈O₁₂

Molecular Weight

462.36

CAS No.

27740-01-8

Solubility (25°C)*

In vitro

DMSO

92 mg/mL (198.97 mM)

In vivo (Add solvents to the product individually and in order)

Clear solution

5%DMSO 1 Corn oil

1.67mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 33.3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Scutellarin (Breviscapine, Breviscapin, Scutellarein-7-glucuronide), the major active principal flavonoids extracted from the Chinese herbal medicines Scutellaria baicalensis and Erigeron breviscapus (Vant.) Hand-Mazz, has many pharmacological effects, such as antioxidant, antitumor, antiviral, and antiinflammatory activities. Scutellarin can down-regulates the STAT3/Girdin/Akt signaling in HCC cells, and inhibits RANKL-mediated MAPK and NF-κB signaling pathway in osteoclasts.

Targets

STAT3 ^[6]

Akt ^[6]

NF-κB ^[7]

In vitro

Scutellarin protects PC12 cells against H2O2-induced cytotoxicity. It attenuates H2O2-induced intracellular accumulation of ROS and lipid peroxidation^[3]. Scutellarin shows negligible inhibitory effects on the six major CYP isoenzymes in human/rat liver microsomes with almost all of the IC50 values exceeding 100 μM, whereas it shows values of 63.8 μM for CYP2C19 in human liver microsomes, and 63.1 and 85.6 μM for CYP2C7 and CYP2C79 in rat liver microsomes, respectively. Scutellarin also has weak inhibitory effect on P-gp^[4]. Scutellarin strongly induces MMP-2 activation and mRNA expression in cultured HUVECs in a concentration-dependent manner. It promotes angiogenesis and may form a basis for angiogenic therapy^[5].

In vivo

The pharmacokinetic results indicate that scutellarin undergoes rapid and extensive biotransformation in vivo. After intraperitoneal injection. scutellarin is absorbed rapidly. The relative bioavailability of oral administration is very low (10.67 %±4.78 %)^[1]. Scutellarin has a series of protective effects including dilate blood vessels, improving microcirculation, decreasing the viscosity of blood, reducing the blood platelet count, inhibiting platelet aggregation activity etc^[2].

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines PC12 cells Concentrations 1 and 10 μmol/L Incubation Time 30 min Method Experiments are carried out 24-48 h after the cells are seeded into plates or dishes. To produce an oxidative stress, H2O2 is freshly prepared from a 30% stock solution prior to each experiment. Preincubation of cells with scutellarin is conducted for 30 min before H2O2 is added. Assays for cell viability, ROS, lipid peroxide, and apoptosis are performed at 6 or 12 h after addition of H2O2.
Animal Study:^{^[2]}	Animal Models Male Wistar rats Dosages 0.09, 0.17, 0.35, 0.70, 1.40 mmol/kg Administration oral

Cell Assay:^{^[3]}

Cell lines
PC12 cells
Concentrations
1 and 10 μmol/L
Incubation Time
30 min
Method
Experiments are carried out 24-48 h after the cells are seeded into plates or dishes. To produce an oxidative stress, H2O2 is freshly prepared from a 30% stock solution prior to each experiment. Preincubation of cells with scutellarin is conducted for 30 min before H2O2 is added. Assays for cell viability, ROS, lipid peroxide, and apoptosis are performed at 6 or 12 h after addition of H2O2.

Animal Study:^{^[2]}

Animal Models
Male Wistar rats
Dosages
0.09, 0.17, 0.35, 0.70, 1.40 mmol/kg
Administration
oral

References

+ Expand

Selleck's Scutellarin has been cited by 1 publication

Scutellarin ameliorates osteoarthritis by protecting chondrocytes and subchondral bone microstructure by inactivating NF-κB/MAPK signal transduction [ Biomed Pharmacother, 2022, 155:113781]	PubMed: 36271561

Scutellarin ameliorates osteoarthritis by protecting chondrocytes and subchondral bone microstructure by inactivating NF-κB/MAPK signal transduction [ Biomed Pharmacother, 2022, 155:113781]

PubMed: 36271561

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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