SC79

Catalog No.S7863 Batch:S786303

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Technical Data

Formula

C17H17ClN2O5
Molecular Weight 364.78 CAS No. 305834-79-1
Solubility (25°C)* In vitro DMSO 72 mg/mL (197.37 mM)
Ethanol 72 mg/mL (197.37 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
3.6mg/ml Taking the 1 mL working solution as an example, add 50 μL of 72 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.
Targets
Akt [1]
In vitro

SC79 suppresses PHAKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells. SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. [1] SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells. [2]
In vivo

In the permanent focal cerebral ischemia mouse model, SC79 (0.04 mg/g, i.p.) enables the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival. [1]

Protocol (from reference)

Kinase Assay:

[1]
  • Cytosolic phosphorylation of Akt

    Hela cells are serum starved for 1 hr and treated with IGF (100ng/mL) or SC79 (4 μg/mL) for 30 minutes. Cells are lysed in Lysis buffer containing 250 mM Sucrose, 20 mM HEPES, 10 mM KCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM EGTA supplemented with protease inhibitors. Cells are passed through 25G needle several times and kept on ice for 20 minutes. Total cell lysate is taken at this point. Cell lysates are centrifuged at 100,000g for 30 minutes. Supernatant is collected as the cytosolic fraction. Pellet is washed with lysis buffer and represents the membrane fraction. Total cell lysate, cytosolic and membrane fractions are resolved by SDS-PAGE and analyzed for phospho-Akt (S473), Total Akt, Tubulin (cytosolic marker) and Orai1 (membrane marker) by western blotting.

Cell Assay:

[1]
  • Cell lines

    HsSultan and NB4 cells

  • Concentrations

    8 μg/mL

  • Incubation Time

    24 h

  • Method

    HsSultan or NB4 cells (2.5 × 105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.
Animal Study:

[1]
  • Animal Models

    Permanent focal cerebral ischemia mouse model

  • Dosages

    0.04 mg/g

  • Administration

    i.p.

Customer Product Validation

Data from [Data independently produced by , , J Exp Clin Cancer Res, 2018, 37(1):41]

Data from [Data independently produced by , , J Exp Clin Cancer Res, 2017, 36(1):105]

Data from [Data independently produced by , , EBioMedicine, 2018, 34:35-45]

Data from [Data independently produced by , , Biochim Biophys Acta Mol Basis Dis, 2017, 1863(11):2848-2861]

Selleck's SC79 has been cited by 158 publications

A pathological joint-liver axis mediated by matrikine-activated CD4+ T cells [ Signal Transduct Target Ther, 2024, 9(1):109] PubMed: 38714712
A pathological joint-liver axis mediated by matrikine-activated CD4+ T cells [ Signal Transduct Target Ther, 2024, 9(1):109] PubMed: 38714712
Combined inhibition of HER2 and VEGFR synergistically improves therapeutic efficacy via PI3K-AKT pathway in advanced ovarian cancer [ J Exp Clin Cancer Res, 2024, 43(1):56] PubMed: 38403634
Jiawei Buzhong Yiqi Decoction attenuates polycystic ovary syndrome through regulating kisspeptin-GPR54-AKT-SHBG system [ Phytomedicine, 2024, 133:155931] PubMed: 39116604
A novel DDIT3 activator dehydroevodiamine effectively inhibits tumor growth and tumor cell stemness in pancreatic cancer [ Phytomedicine, 2024, 155377] PubMed: none
Aberrant methylation and expression of TNXB promote chondrocyte apoptosis and extracullar matrix degradation in hemophilic arthropathy via AKT signaling [ Elife, 2024, 13RP93087] PubMed: 38819423
Dysregulation of mTOR signaling mediates common neurite and migration defects in both idiopathic and 16p11.2 deletion autism neural precursor cells [ Elife, 2024, 13e82809] PubMed: 38525876
Aberrant methylation and expression of TNXB promote chondrocyte apoptosis and extracullar matrix degradation in hemophilic arthropathy via AKT signaling [ Elife, 2024, 13RP93087] PubMed: 38819423
Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling [ Genes Dis, 2024, 11(2):964-977] PubMed: 37692479
CBX1 is involved in hepatocellular carcinoma progression and resistance to sorafenib and lenvatinib via IGF-1R/AKT/SNAIL signaling pathway [ Hepatol Int, 2024, 10.1007/s12072-024-10696-0] PubMed: 38769286

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.