Astragaloside IV

Catalog No.S3901 Batch:S390102

Technical Data

Formula	C₄₁H₆₈O₁₄
Molecular Weight	784.97	CAS No.	84687-43-4
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (127.39 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Astragaloside IV (AST-IV, AS-IV) is a bioactive saponin first isolated from the dried plant roots of the genus Astragalus, which is used in traditional Chinese medicine. It has various effect on the cardiovascular, immune, digestive, and nervous systems. AS-IV suppresses activation of p-Akt, p-mTOR, p-NF-κB and p-Erk1/2.

Targets

JNK ^[2]

ERK ^[2]

Akt ^[2]

mTOR ^[2]

NF-κB ^[2]

In vitro

Astragaloside IV probably alleviates leptin resistance by comprehensive modulation of ObR/STAT3 signaling transduction in neuronal cells^[2].

In vivo

Administration of astragaloside IV (AS-IV) reduces albuminuria, ameliorates changes in the glomerular and tubular pathology, and decreases urinary NAG, NGAL, and TGF-β1 in db/db mice. AS-IV also attenuates the diabetes-related activation of Akt/mTOR, NFκB, and Erk1/2 signaling pathways without causing any detectable hepatotoxicity. AS-IV lowers blood glucose levels in streptozotocin (STZ)-induced diabetic models. It prevents body weight gain and reduced accumulation of fat tissues in HFD fed mice. Astragaloside IV actively participates in the modulation of energy homeostasis^[1]^[2].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines SH-SY5Y cells Concentrations 0, 25, 50 and 100 μM Incubation Time 24 h Method Neuronal cell line SH-SY5Y cells are maintained in DMEM/F-12 medium supplemented with 10% fetal bovine serum (FBS) at 37 °C in a humidified incubator supplied with 5% CO2. After seeded in 6-well plate at 5 × 10⁵ cells/ml and cultured without FBS overnight, the cells are treated with ASI (0, 25, 50 and 100 μM) for 24 h. Thereafter, they are harvested with CelLyticTM MT mammalian tissue lysis reagent supplemented with protease and phosphatase inhibitors followed by sonication, and stored at −80℃ for further western blotting analysis.
Animal Study:^{^[2]}	Animal Models Six-weeks old male C57BL/6 mice Dosages 25 mg/kg/d Administration i.p.

Cell Assay:^{^[2]}

Cell lines
SH-SY5Y cells
Concentrations
0, 25, 50 and 100 μM
Incubation Time
24 h
Method
Neuronal cell line SH-SY5Y cells are maintained in DMEM/F-12 medium supplemented with 10% fetal bovine serum (FBS) at 37 °C in a humidified incubator supplied with 5% CO2. After seeded in 6-well plate at 5 × 10⁵ cells/ml and cultured without FBS overnight, the cells are treated with ASI (0, 25, 50 and 100 μM) for 24 h. Thereafter, they are harvested with CelLyticTM MT mammalian tissue lysis reagent supplemented with protease and phosphatase inhibitors followed by sonication, and stored at −80℃ for further western blotting analysis.

Animal Study:^{^[2]}

Animal Models
Six-weeks old male C57BL/6 mice
Dosages
25 mg/kg/d
Administration
i.p.

References

Selleck's Astragaloside IV has been cited by 4 publications

SUV39H1 Inhibits Angiogenesis in Limb Ischemia of Mice [ Cell Transplant, 2023, 32:9636897231198167]	PubMed: 37811706
SUV39H1 Inhibits Angiogenesis in Limb Ischemia of Mice [ Cell Transplant, 2023, 32:9636897231198167]	PubMed: 37811706
Astragaloside IV attenuate MI-induced myocardial fibrosis and cardiac remodeling by inhibiting ROS/caspase-1/GSDMD signaling pathway [ Cell Cycle, 2022, 1-14]	PubMed: 35770948
Astragaloside IV and echinacoside benefit neuronal properties via direct effects and through upregulation of SOD1 astrocyte function in vitro [ Naunyn Schmiedebergs Arch Pharmacol, 2020, 10.1007/s00210-020-02022-w]	PubMed: 33219470

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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