Sarilumab (anti-IL-6Rα)

Catalog No.A2011        Batch: A201101

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Technical Data

CAS No. 1189541-98-7
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 98.05%
Protein concentration 5.0 mg/ml
Endotoxin Level ≤1 EU/mg

Biological Activity

Description Sarilumab (anti-IL-6Rα) is a fully human anti-IL-6Rα mAb that binds membrane-bound and soluble human IL-6Rα with high affinity, MW: 144.13 KD.
Targets
IL-6R (human) [1] IL-6R (monkey) [1]
61.9 pM(Kd) 71.9 pM(Kd)
In vitro

Sarilumab can reverse IL-6 enhanced colony formation in HCC cells under sorafenib or regorafenib treatment.[1]
In vivo

Sarilumab has been shown in preclinical studies to inhibit IL-6 signalling in a dose-dependent manner[2].

Protocol (Only for Reference)

Cell Assay:

  • Cells were treated with drugs for 36 h. The soluble form of IL-6Rα in cell culture supernatants was measured with a human IL-6Rα ELISA kit according to the manufacturer’s instructions. Plates were measured on a microplate reader at a wavelength of 450 nm.

Selleck's Sarilumab (anti-IL-6Rα) has been cited by 4 publications

Personalized cardiovascular risk assessment in Rheumatoid Arthritis patients using circulating molecular profiles and their modulation by TNFi, IL6Ri, and JAKinibs [ Biomed Pharmacother, 2024, 173:116357] PubMed: 38479179
Human iPSC-Derived Proinflammatory Macrophages cause Insulin Resistance in an Isogenic White Adipose Tissue Microphysiological System [ Small, 2023, e2203725.] PubMed: 37104853
Induction of IL-6Rα by ATF3 enhances IL-6 mediated sorafenib and regorafenib resistance in hepatocellular carcinoma [ Cancer Lett, 2022, 524:161-171] PubMed: 34687791
Human mesenchymal stem cells-derived conditioned medium inhibits hypoxia-induced death of neonatal porcine islets by inducing autophagy. [ Xenotransplantation, 2019,  doi: 10] PubMed: 31578787

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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