Saquinavir (Ro 31-8959)

Catalog No.S9625 Batch:S962501

Technical Data

Formula	C₃₈H₅₀N₆O₅
Molecular Weight	670.84	CAS No.	127779-20-8
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (149.06 mM)
		Ethanol	25 mg/mL (37.26 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Saquinavir (Ro 31-8959,SAQ) is a potent and selective inhibitor of HIV proteinase with a mean IC50 of 2.7 nM in JM cells.

Targets

HIV proteinase ^[1]
(in JM cells)

2.7 nM

In vitro

Saquinavir (Ro 31-8959) is an effective selective HIV protease inhibitor with an average IC50 of 2.7 nM in JM cells, which inhibits the spread of HIV infection and the production of cytopathic effects.<sup><a class="sref" href="#s_ref">[1]</a></sup>

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines JM cells Concentrations 0.5-100 nM Incubation Time 3 days Method JM cells (2 × 10⁵ cells/ml) were exposed to HIV-1 (GB8) at a multiplicity of infection (MOI) of 0.01 syncytium-forming units/cell. Aliquots (0.2 ml) of cells were placed in microtitre wells with appropriate dilutions of Ro 31-8959 added to 5 replicate cultures. After incubation for 3 days at 37°C in a humidified atmosphere of 5% CO2 in air, syncytia were counted.

Cell Assay:^{^[1]}

Cell lines
JM cells
Concentrations
0.5-100 nM
Incubation Time
3 days
Method
JM cells (2 × 10⁵ cells/ml) were exposed to HIV-1 (GB8) at a multiplicity of infection (MOI) of 0.01 syncytium-forming units/cell. Aliquots (0.2 ml) of cells were placed in microtitre wells with appropriate dilutions of Ro 31-8959 added to 5 replicate cultures. After incubation for 3 days at 37°C in a humidified atmosphere of 5% CO2 in air, syncytia were counted.

References

[1] J C Craig, et al. Antiviral Res . 1991 Dec;16(4):295-305.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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