Salvianolic acid B

Catalog No.S4735 Batch:S473501

Technical Data

Formula	C₃₆H₃₀O₁₆
Molecular Weight	718.61	CAS No.	121521-90-2
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (139.15 mM)
		Water	100 mg/mL (139.15 mM)
		Ethanol	100 mg/mL (139.15 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Salvianolic acid B (Sal B, Lithospermate B, Lithospermic acid B), an antioxidant and free radical scavenging compound, is the most abundant bioactive compound extracted from the root of Salvia miltiorrhiza Bunge.

Targets

SIRT ^[2]

In vitro

Salvianolic acid B, also known as satanic acid B or lithospermic acid B, is a new generation of the natural antioxidants. It can influence Ca2+ aggregation and endothelial cell NO release of hypoxia/ reoxygenation-induced cell. When acid B concentration is 2.5, 5, and 10 mg/l, cell viability and superoxide dismutase (SOD) activity are enhanced, and the formation of malondialdehyde (MDA) in human umbilical vein endothelial cells (ECV304) is inhibited. SalB inhibits HG-induced oxidative stress and reduces the generation of ROS and 8-hydroxy-2-deoxyguanosine (8-OHDG) and mitochondrial depolarization and apoptosis in a dose-dependent manner. It can downregulate the expression of Bax and AIF nuclear translocation and cytochrome c release mediated by HG, but upregulate the expression of Bcl-2 induced by HG. Besides, SalB attenuates HG-induced caspase of the enzyme 3, 9 and minimize PARP cleavage of Schwann cells (SCs). SalB inhibits angiotensin II or H2O2 and TNF-α-induced gelatinolytic activity in human aortic smooth muscle cells (HASMCs) in a concentration-dependent manner. Salvianolic acid B can inhibit platelet aggregation and adhesion. Salvianolic acid B can promote cardiac angiogenesis effect. SalB can enhance cell activity and reduce the number of sub-G1 and apoptotic nuclei of ischemic cell model in order to show its antiapoptotic effects. Salvianolic acid B inhibits ischemia and hypoxia of myocardial injury. Salvianolic acid B inhibits the synthesis of type I collagen of non-TGF-1 stimulated human hepatic stellate cell line (LX-2)^[1]. SalB activates mammalian sirtuins 1 (SIRT1), an NAD-dependent class III histone deacetylase (HDAC) that plays important roles in several physiological processes, including gene transcription, senescence, energy metabolism, oxidative stress and inflammation^[2]. (HG：High glucose)

In vivo

Salvianolic acid B can significantly reduce the myocardial infarct size and blood lactate dehydrogenase level of model rat with acute myocardial infarction, improve cardiac function and myocardial tissue structure, thus inhibiting ischemia and hypoxia of myocardial injury. salvianolic acid B can improve blood hemorheology, reduce oxidative damage, improve the vascular endothelial cell function, and prevent the development of coronary artery disease. Salvianolic acid B could selectively inhibit the activity of MMP-9 in a rat model of myocardial infarction. Salvianolic acid B can also effectively increase the thickness of the left ventricular wall in the myocardial infarction rats to improve the contraction of the heart, and reduce cardiac fibrosis^[1]. SalB treatment ameliorates ethanol-induced hepatic inflammation by decreasing the levels of hepatotoxic cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). SalB has beneficial effects against hepatic fibrosis in animal models and has been shown to possess cardioprotective and neuroprotective activity via anti-oxidative and anti-inflammatory actions^[2].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines The HepG2 human hepatoma cell line Concentrations 8 μM Incubation Time 3 h Method The HepG2 human hepatoma cell line is cultured in MEM containing 10% (v/v) FBS. The cells are incubated at 37℃ in humidified air with 5% CO2. HepG2 cells are seeded at a density of 1×10_⁵ cells per well and grown for 24 h. After this, the cells are treated with 8 μM SalB for 3 h or 10 mM Ex527 or RES for 6 h. Then, the cells are exposed to 100 mM ethanol for 48 h.
Animal Study:^{^[2]}	Animal Models Male Sprague-Dawley rats Dosages 15 or 30 mg/kg/d Administration intragastric administration

Cell Assay:^{^[2]}

Cell lines
The HepG2 human hepatoma cell line
Concentrations
8 μM
Incubation Time
3 h
Method
The HepG2 human hepatoma cell line is cultured in MEM containing 10% (v/v) FBS. The cells are incubated at 37℃ in humidified air with 5% CO2. HepG2 cells are seeded at a density of 1×10_⁵ cells per well and grown for 24 h. After this, the cells are treated with 8 μM SalB for 3 h or 10 mM Ex527 or RES for 6 h. Then, the cells are exposed to 100 mM ethanol for 48 h.

Animal Study:^{^[2]}

Animal Models
Male Sprague-Dawley rats
Dosages
15 or 30 mg/kg/d
Administration
intragastric administration

References

Selleck's Salvianolic acid B has been cited by 6 publications

Xuebijing improves intestinal microcirculation dysfunction in septic rats by regulating the VEGF-A/PI3K/Akt signaling pathway [ World J Emerg Med, 2024, 15(3):206-213]	PubMed: 38855370
SUV39H1 Inhibits Angiogenesis in Limb Ischemia of Mice [ Cell Transplant, 2023, 32:9636897231198167]	PubMed: 37811706
SUV39H1 Inhibits Angiogenesis in Limb Ischemia of Mice [ Cell Transplant, 2023, 32:9636897231198167]	PubMed: 37811706
Salvianolic acid-B improves fat graft survival by promoting proliferation and adipogenesis [ Stem Cell Res Ther, 2021, 12(1):507]	PubMed: 34535194
Salvianolic acid-B improves fat graft survival by promoting proliferation and adipogenesis [ Research Square, 2021, 10.21203/rs.3.rs-372611/v1]	PubMed: None
Salvianolic acid B inhibits ototoxic drug-induced ototoxicity by suppression of the mitochondrial apoptosis pathway. [ J Cell Mol Med, 2020, 29]	PubMed: 32351026

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SHIPPING AND STORAGE
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