Dantrolene sodium

Catalog No.S5478 Batch:S547803

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Technical Data

Formula

C14H9N4NaO5
Molecular Weight 336.23 CAS No. 14663-23-1
Solubility (25°C)* In vitro DMSO 10 mg/mL (29.74 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
0.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Dantrolene sodium(F 440) acts as a postsynaptic muscle relaxant by inhibiting Ca2+ ions release from sarcoplasmic reticulum stores by antagonizing ryanodine receptors.
In vitro

Treatment with a mixture of Thaps. and dantrolene during optogenetic stimulation (OS) of Schwann cells (SCs) significantly decreases the percentage of MBP-expressing SCs and, importantly, reduces the percentage of MBP-expressing SCs. Mixture of Thaps. and dantrolene decreases drastically the ΔF/F value in SCs.[1]
In vivo

Dantrolene administration decreases plasma GLuc-SERCaMP activity and endogenous SERCaMP activity when compared to vehicle during ad lib high fat diet. Rats administered dantrolene gains less weight compared to vehicle treated rats, and have less caloric intake.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Schwann cells

  • Concentrations

    600 nM

  • Incubation Time

    4 h

  • Method

    Thaps. + Dantrolene (600 nM, a ryanodine receptor antagonist) for 4 h prior to examining the intracellular Ca2+ levels of SCs. Representative inverted images of BABTA-1 AM fluorescence signals showing the intracellular Ca2+ on SCs.

Selleck's Dantrolene sodium has been cited by 4 publications

Porcine reproductive and respiratory syndrome virus infection triggers autophagy via ER stress-induced calcium signaling to facilitate virus replication [ PLoS Pathog, 2023, 19(3):e1011295] PubMed: 36972295
Heliox Preconditioning Exerts Neuroprotective Effects on Neonatal Ischemia/Hypoxia Injury by Inhibiting Necroptosis Induced by Ca2+ Elevation [ Transl Stroke Res, 2022, 10.1007/s12975-022-01021-8] PubMed: 35445968
Reactive Oxygen Species Regulate Endoplasmic Reticulum Stress and ER-Mitochondrial Ca2+ Crosstalk to Promote Programmed Necrosis of Rat Nucleus Pulposus Cells under Compression [ Oxid Med Cell Longev, 2021, 2021:8810698] PubMed: 33815661
Optogenetic stimulation promotes Schwann cell proliferation, differentiation, and myelination in vitro. [ Sci Rep, 2019, 9(1):3487] PubMed: 30837563

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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