Rucaparib Camsylate

Catalog No.S5195 Batch:S519501

Technical Data

Formula	C₂₉H₃₄FN₃O₅S
Molecular Weight	555.66	CAS No.	1859053-21-6
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (179.96 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Rucaparib (Rubraca, AG014699, PF01367338) Camsylate is an inhibitor of PARP with Ki of 1.4 nM for PARP1 in a cell-free assay, also showing binding affinity to eight other PARP domains.

Targets

PARP1 ^[1]
(Cell-free assay)

1.4 nM(Ki)

In vitro

Deletion of ATM does not impact sensitivity to PARP inhibition by rucaparib.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Human prostate cancer lines DU145, ATM-deficient isogenic DU145 cells Concentrations 0-5 µM Incubation Time 3-4 days Method Cells were seeded into 24-well plates (2,500–4000 cells/well) and treated with increasing concentrations of drug the following day. Cell viability was assessed after 72–96 hours by adding CellTiter-Glo reagent and measuring luminescence using a plate reader. For clonogenic survival assays, cells were seeded in 6-well plates (500–4000 cells/well) in triplicate. Cells were treated with drug 16 to 18 hours after plating and were allowed to grow for 14 days. (Data sourced from selleck products)

Cell Assay:^{^[2]}

Cell lines
Human prostate cancer lines DU145, ATM-deficient isogenic DU145 cells
Concentrations
0-5 µM
Incubation Time
3-4 days
Method
Cells were seeded into 24-well plates (2,500–4000 cells/well) and treated with increasing concentrations of drug the following day. Cell viability was assessed after 72–96 hours by adding CellTiter-Glo reagent and measuring luminescence using a plate reader. For clonogenic survival assays, cells were seeded in 6-well plates (500–4000 cells/well) in triplicate. Cells were treated with drug 16 to 18 hours after plating and were allowed to grow for 14 days.

(Data sourced from selleck products)

References

Selleck's Rucaparib Camsylate has been cited by 14 publications

Vanguard is a Glucose Deprivation-Responsive Long Non-Coding RNA Essential for Chromatin Remodeling-Reliant DNA Repair [ Adv Sci (Weinh), 2022, 9(30):e2201210]	PubMed: 36047643
Inhibition of BAD-Ser99 phosphorylation synergizes with PARP inhibition to ablate PTEN-deficient endometrial carcinoma [ Cell Death Dis, 2022, 13(6):558]	PubMed: 35725817
Cancer Cells Haploinsufficient for ATM Are Sensitized to PARP Inhibitors by MET Inhibition [ Int J Mol Sci, 2022, 23(10)5770]	PubMed: 35628590
Combined inhibition of BADSer99 phosphorylation and PARP ablates models of recurrent ovarian carcinoma [ Commun Med (Lond), 2022, 2:82]	PubMed: 35791346
Chemoresistance is mediated by ovarian cancer leader cells in vitro [ J Exp Clin Cancer Res, 2021, 40(1):276]	PubMed: 34470672
BKM120 sensitizes glioblastoma to the PARP inhibitor rucaparib by suppressing homologous recombination repair [ Cell Death Dis, 2021, 12(6):546]	PubMed: 34039959
Pharmacological Poly (ADP-Ribose) Polymerase Inhibitors Decrease Mycobacterium tuberculosis Survival in Human Macrophages [ Front Immunol, 2021, 12:712021]	PubMed: 34899683
Synergistic targeting of BRCA1 mutated breast cancers with PARP and CDK2 inhibition [ NPJ Breast Cancer, 2021, 7(1):111]	PubMed: 34465787
Role of pyruvate in maintaining cell viability and energy production under high-glucose conditions [ Sci Rep, 2021, 11(1):18910]	PubMed: 34556698
Poly(ADP-Ribose) Polymerase Inhibitors for Arsenic Trioxide-Resistant Acute Promyelocytic Leukemia: Synergistic In Vitro Antitumor Effects with Hypomethylating Agents or High-Dose Vitamin C [ J Pharmacol Exp Ther, 2021, 377(3):385-397]	PubMed: 33820831

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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