RSL3

Catalog No.S8155 Batch:S8155

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Technical Data

Formula

C23H21ClN2O5
Molecular Weight 440.88 CAS No. 1219810-16-8
Solubility (25°C)* In vitro DMSO 88 mg/mL (199.6 mM)
Water Insoluble
Ethanol '''22 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.
Targets
Gpx4 [1]
(In Calu-1 cells)
In vitro Ferroptosis-inducing compounds inactivate GPX4 by direct binding or by depleting glutathione.After binding to GPX4, RSL3 inactivates GPX4 to induce ROS production from lipid peroxidation. RSL3's ability to induce synthetic lethality with oncogenic RAS is rapid and quite potent. This compound inhibits the growth of BJ-TERT/LT/ST/RASV12 and DRD cells as low as 10 ng/mland started to kill sensitive cells as early as 8 hr after treatment[1][2].
In vivo Prostaglandin-endoperoxide synthase (PTGS) is the key enzyme in prostaglandin biosynthesis. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2. PTGS2 encoding cyclooxygenase-2 (COX-2) is significantly upregulated after treatment with RSL3 and erastin in mice[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    TERT/LT/ST/RASV12 cells 

  • Concentrations

    1 µg/ml

  • Incubation Time

    16hrs

  • Method

    TERT/LT/ST/RASV12 cells are seeded in 10 cm dishes and treated with 1 µM staurosporine, 10 µg/ml erastin, 20 µg/ml RSL5, and 1 µg/ml RSL3 for 16 hr. Both dying cells and live cells in each 10 cm dish are harvested and collected in the same 15 ml tubes by centrifuging cell suspension at 1000 rpm for 5 min.
Animal Study:

[1]
  • Animal Models

    athymic nude mice(8 weeks)implanted with subcutaneous xenograft tumors derived from BJeLR cells

  • Dosages

    100 mg/kg

  • Administration

    s.c. injection

Customer Product Validation

, , Am J Physiol Renal Physiol, 2017, doi: 10.1152/ajprenal.00044.2017

Data from [Data independently produced by , , Nature, 2017, 547(7664):453-457]

Data from [Data independently produced by , , Cell, 2017, 171(3):628-641]

Data from [Data independently produced by , , Cell Res, 2016, 26(9):1021-32]

Selleck's RSL3 has been cited by 422 publications

Autophagy regulator ATG5 preserves cerebellar function by safeguarding its glycolytic activity [ Nat Metab, 2025, 7(2):297-320] PubMed: 39815080
Regulation of enzymatic lipid peroxidation in osteoblasts protects against postmenopausal osteoporosis [ Nat Commun, 2025, 16(1):758] PubMed: 39824794
S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism [ Nat Commun, 2025, 16(1):509] PubMed: 39779666
Targeting pancreatic cancer glutamine dependency confers vulnerability to GPX4-dependent ferroptosis [ Cell Rep Med, 2025, 6(2):101928] PubMed: 39879992
Regulation of calcium signaling prevents neuronal death mediated by NIST DEP in xenoferroptotic cell death conditions [ J Hazard Mater, 2025, 488:137374] PubMed: 39892142
SLC25A1 and ACLY maintain cytosolic acetyl-CoA and regulate ferroptosis susceptibility via FSP1 acetylation [ EMBO J, 2025, 10.1038/s44318-025-00369-5] PubMed: 39881208
Inhibition of KLF5 promotes ferroptosis via the ZEB1/HMOX1 axis to enhance sensitivity to oxaliplatin in cancer cells [ Cell Death Dis, 2025, 16(1):28] PubMed: 39827156
Acyl-CoA thioesterase 8 induces gemcitabine resistance via regulation of lipid metabolism and antiferroptotic activity in pancreatic ductal adenocarcinoma [ Acta Pharmacol Sin, 2025, 10.1038/s41401-025-01477-y] PubMed: 39939803
Downregulation of the SREBP pathways and disruption of redox status by 25-hydroxycholesterol predispose cells to ferroptosis [ Free Radic Biol Med, 2025, 228:319-328] PubMed: 39778605
Targeting oxidative stress-induced lipid peroxidation enhances podocyte function in cystinosis [ J Transl Med, 2025, 23(1):206] PubMed: 39980044

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.