Rosmarinic acid

Catalog No.S3612 Batch:S361201

Technical Data

Formula	C₁₈H₁₆O₈
Molecular Weight	360.31	CAS No.	20283-92-5
Solubility (25°C)*	In vitro	DMSO	72 mg/mL (199.82 mM)
		Ethanol	72 mg/mL (199.82 mM)
		Water	24 mg/mL (66.6 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Rosmarinic acid (RA, Rosemary acid) is a naturally occurring hydroxylated compound. It has the ability to block complement fixation, inhibit lipoxygenase and cyclooxygenase activity and inhibit the expression of CCL11 and CCR3 by suppressing the IKK-β activity in NF-κB activation signaling.

Targets

IKK β ^[2]
(cell-based assay)

12 μM

In vitro

RA exerts a significant cytoprotective effect by scavenging intracellular ROS induced by UVB. RA also attenuates UVB-induced oxidative macromolecular damage, including protein carbonyl content, DNA strand breaks, and the level of 8-isoprostane. Furthermore, RA increases the expression and activity of superoxide dismutase, catalase, heme oxygenase-1, and their transcription factor Nrf2, which are decreased by UVB radiation. RA treatment recovers the protein expression levels of Nrf2 decreased by UVB exposure, and results in the translocation of Nrf2 protein from the cytosol into the nucleus. RA may protect cellular environments from free-radical damage and thereby, enhance the cellular antioxidant defense system^[1]. Rosmarinic acid inhibits TNF-α-induced phosphorylation and degradation of IκB-α, as well as nuclear translocation of NF-κB heterodimer induced by TNF-α. It downregulates the expression of CCL11 and CCR3 via the inhibition of NF-κB activation signaling. Rosmarinic acid inhibits the Ca2+-dependent pathways of TCR-mediated signaling by inhibiting PLC-γ1 and Itk activities^[2].

In vivo

RA improves histological and serum markers of liver damage and significantly ameliorates oxidative/nitrosative stress and inflammatory response in liver tissue. Additionally, RA prevents transforming growth factor-beta1 (TGF-b1) and alpha-smooth muscle actin (a-SMA) expression, suggesting suppression of profibrotic response. RA significantly inhibits the CCl4-induced apoptosis, which is evident from decreased cleavage of caspase-3. The hepatoprotective activity of RA coincides with enhanced NF-E2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1) expression. Rosmarinic acid acts as a natural inducer of endogenous cellular antioxidant defense via activation of the Nrf2/HO-1 pathway^[3].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HaCaT cells Concentrations 0.625, 1.25, 2.5, or 5 μM Incubation Time 48 h Method Cells are treated with RA (0.625, 1.25, 2.5, or 5 μM) and exposed to UVB radiation 1 h later. They are then incubated at 37°C for 48 h. At this time, MTT is added to each well to obtain a total reaction volume of 200 μl. After 4 h incubation, the supernatant is removed by aspiration. The formazan crystals in each well are dissolved in dimethyl sulfoxide (DMSO; 150 μl), and the absorbance at 540 nm is measured on a scanning multi-well spectrophotometer.
Animal Study:^{^[3]}	Animal Models Male BALB/cN mice Dosages 10, 25 and 50 mg/kg Administration by gavage

Cell Assay:^{^[1]}

Cell lines
HaCaT cells
Concentrations
0.625, 1.25, 2.5, or 5 μM
Incubation Time
48 h
Method
Cells are treated with RA (0.625, 1.25, 2.5, or 5 μM) and exposed to UVB radiation 1 h later. They are then incubated at 37°C for 48 h. At this time, MTT is added to each well to obtain a total reaction volume of 200 μl. After 4 h incubation, the supernatant is removed by aspiration. The formazan crystals in each well are dissolved in dimethyl sulfoxide (DMSO; 150 μl), and the absorbance at 540 nm is measured on a scanning multi-well spectrophotometer.

Animal Study:^{^[3]}

Animal Models
Male BALB/cN mice
Dosages
10, 25 and 50 mg/kg
Administration
by gavage

References

Selleck's Rosmarinic acid has been cited by 1 publication

Rosmarinic acid prevents refractory bacterial pneumonia through regulating Keap1/Nrf2-mediated autophagic pathway and mitochondrial oxidative stress [ Free Radic Biol Med, 2021, S0891-5849(21)00198-2]	PubMed: 33812997

Rosmarinic acid prevents refractory bacterial pneumonia through regulating Keap1/Nrf2-mediated autophagic pathway and mitochondrial oxidative stress [ Free Radic Biol Med, 2021, S0891-5849(21)00198-2]

PubMed: 33812997

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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