Rhamnose

Catalog No.E0245 Batch:E024501

Technical Data

Formula	C₆H₁₂O₅
Molecular Weight	164.16	CAS No.	3615-41-6
Solubility (25°C)*	In vitro	DMSO	33 mg/mL (201.02 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Rhamnose(L-Rhamnose) is an important 6-deoxy sugar present in many natural products, glycoproteins, and structural polysaccharides.
In vitro	The polymeric copies of a rhamnose motif form glycopolymers (ARGPs), which can bind endogenous antirhamnose antibodies present in human serum, thus attract antibodies from human serum to the target cell surface.^[1]
In vivo	Rhamnose(Rha) conjugated immunogens induces high titers of anti-Rha antibodies in wildtype mice, which can replace α1,3galactosyltransferase knockout (α1,3GT KO) mice in such antigen/antibody-mediated vaccine design when developing cancer immunotherapies.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Jurkat T Cells Concentrations 1 mg/mL Incubation Time 1 h Method Jurkat T cells are resuspended in the RPMI medium without FBS at a final concentration of 350000 cells/mL and placed on ice. After 30 min of incubation on ice, 1 mL of cold cell suspension is incubated for 1 h on ice in the dark with 40 μL of a 1 mg/mL stock solution of the CholA-p(RhamEAmAIBN) polymer in PBS. As a negative control, 1 mL of cold cell suspension is incubated for 1 h on ice in the dark with 40 μL of a 1 mg/mL stock solution of the CholA-p(GalEAmAIBN) polymer in PBS. After the incubation, cells are washed twice with PBS by centrifugation for 3 min at 500 G. Afterward, cells are resuspended in 500 μL of flow incubation buffer (1% bovine serum albumin, 5 mM Na2EDTA in PBS) containing 10% of human serum and are then incubated for 30 min in the dark on ice. After this incubation period, cells are centrifuged at 500 G for 3 min and washed once with PBS. Cells are spun down again at 500 G for 3 min and incubated during 1 h in the dark at room temperature with 500 μL of goat antihuman IgG secondary antibody bound to Alexa 647 at a final concentration of 4 μL/mL and 1% human albumin serum in PBS. Incubation is followed by two washing steps with PBS by centrifugation at 500 G for 5 min. Cells are imaged using stochastic optical reconstruction microscopy.
Animal Study:^{^[2]}	Animal Models BALB/c mice Dosages 30 µg Administration s.c.

Cell Assay:^{^[1]}

Cell lines
Jurkat T Cells
Concentrations
1 mg/mL
Incubation Time
1 h
Method
Jurkat T cells are resuspended in the RPMI medium without FBS at a final concentration of 350000 cells/mL and placed on ice. After 30 min of incubation on ice, 1 mL of cold cell suspension is incubated for 1 h on ice in the dark with 40 μL of a 1 mg/mL stock solution of the CholA-p(RhamEAmAIBN) polymer in PBS. As a negative control, 1 mL of cold cell suspension is incubated for 1 h on ice in the dark with 40 μL of a 1 mg/mL stock solution of the CholA-p(GalEAmAIBN) polymer in PBS. After the incubation, cells are washed twice with PBS by centrifugation for 3 min at 500 G. Afterward, cells are resuspended in 500 μL of flow incubation buffer (1% bovine serum albumin, 5 mM Na2EDTA in PBS) containing 10% of human serum and are then incubated for 30 min in the dark on ice. After this incubation period, cells are centrifuged at 500 G for 3 min and washed once with PBS. Cells are spun down again at 500 G for 3 min and incubated during 1 h in the dark at room temperature with 500 μL of goat antihuman IgG secondary antibody bound to Alexa 647 at a final concentration of 4 μL/mL and 1% human albumin serum in PBS. Incubation is followed by two washing steps with PBS by centrifugation at 500 G for 5 min. Cells are imaged using stochastic optical reconstruction microscopy.

Animal Study:^{^[2]}

Animal Models
BALB/c mice
Dosages
30 µg
Administration
s.c.

References

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SHIPPING AND STORAGE
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