RGD peptide (GRGDNP)

Catalog No.S4513 Batch:S451303

Technical Data

Formula	C₂₃H₃₈N₁₀O₁₀
Molecular Weight	614.61	CAS No.	114681-65-1
Solubility (25°C)*	In vitro	Water	100 mg/mL (162.7 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

RGD peptide (GRGDNP) is an inhibitor of binding of integrins to the extracellular matrixs. RGD peptide (GRGDNP) induces apoptosis presumably through direct activation of caspase-3.

Targets

integrin ^[1]

caspase-3 ^[2]

In vitro

RGD-peptide interfers with cell proliferation by affecting cell protrusion and stress fibers.^[3]

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines MC3T3-E1: ATCC CRL-2594 cell line Concentrations 1,000 µM Incubation Time 24 h Method For inhibition of cell attachment, MC3T3-E1 cells were incubated at 37°C for 24 hours in the presence of 5 mM EDTA, 1,000 µM RGD, 10 µg/mL MAB1998, 0.2 µL IgG, and 10 µg/mL heparin. Next, the cells (500 µL, 5,000 cells/well) were added to the wells and incubated for 24 hours. To measure cell proliferation on the specimens, a metabolic activity cell counting kit assay was used in this experiment. (Data sourced from selleck products)

Cell Assay:^{^[3]}

Cell lines
MC3T3-E1: ATCC CRL-2594 cell line
Concentrations
1,000 µM
Incubation Time
24 h
Method
For inhibition of cell attachment, MC3T3-E1 cells were incubated at 37°C for 24 hours in the presence of 5 mM EDTA, 1,000 µM RGD, 10 µg/mL MAB1998, 0.2 µL IgG, and 10 µg/mL heparin. Next, the cells (500 µL, 5,000 cells/well) were added to the wells and incubated for 24 hours. To measure cell proliferation on the specimens, a metabolic activity cell counting kit assay was used in this experiment.

(Data sourced from selleck products)

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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