RGD (Arg-Gly-Asp) Peptides

Catalog No.S8008 Batch:S800804

Technical Data

Formula	C₁₂H₂₂N₆O₆
Molecular Weight	346.34	CAS No.	99896-85-2
Solubility (25°C)*	In vitro	Water	69 mg/mL (199.22 mM)
		DMSO	7 mg/mL (20.21 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

RGD (Arg-Gly-Asp) Peptides is a cell adhesion motif which can mimic cell adhesion proteins and bind to integrins.

Targets

Integrin ^[1]

In vitro

The RGD sequence is the cell attachment site of a large number of adhesive extracellular matrix, blood, and cell surface proteins, and nearly half of the over 20 known integrins recognize this sequence in their adhesion protein ligands. The RGD peptides and mimics can be used to probe integrin functions in various biological systems. Drug design based on the RGD structure may provide new treatments for diseases such as thrombosis, osteoporosis, and cancer. ^[1] RGD peptide acts as an inhibitor of integrin-ligand interactions and can induce apoptosis in the absence of signals and integrin-mediated cell clustering. Research demonstrates that RGD peptides promote apoptosis through activation of conformation changes that enhance pro-caspase-3 activation and autoprocessing. ^[2] The RGD peptide can serve as a cell adhesion site of extracellular matrix, cell surface proteins, and integrins. ^[3] In addition, RGD peptide can inhibit ACK-2 activation through cell adhesion. ^[4]

Protocol (from reference)

Cell Assay:^{^[5]}	Cell lines HLE cell line Concentrations 0.1, 0.3, 1.0 and 2.0 mg/ml Incubation Time 90 min Method The cells were seeded at approximately 2.6 × 10⁴ /well on each ECM-coated 24-well plate in the medium containing the monotype of GRGDSP RGD peptide at 0 (control), 0.1, 0.3, 1.0 and 2.0 mg/ml. After 90 min of incubation, the floating cells were removed by washing twice with Ca 2+ -Mg 2+ -free phosphate-buffered saline. Then the attached cells were dissociated with trypsin-EDTA solution. The number of cells attached at each concentration of peptide was counted using a hemocytometer.

Cell Assay:^{^[5]}

Cell lines
HLE cell line
Concentrations
0.1, 0.3, 1.0 and 2.0 mg/ml
Incubation Time
90 min
Method
The cells were seeded at approximately 2.6 × 10⁴ /well on each ECM-coated 24-well plate in the medium containing the monotype of GRGDSP RGD peptide at 0 (control), 0.1, 0.3, 1.0 and 2.0 mg/ml. After 90 min of incubation, the floating cells were removed by washing twice with Ca 2+ -Mg 2+ -free phosphate-buffered saline. Then the attached cells were dissociated with trypsin-EDTA solution. The number of cells attached at each concentration of peptide was counted using a hemocytometer.

References

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Customer Product Validation

: Data from [Data independently produced by , , Int J Nanomedicine, 2018, 13:7657-7667]

: Data from [Data independently produced by , , J Biol Chem, 2016, 291(28):14815-25.]

: Data from [Data independently produced by , , Neuroscience, 2019, 396:187-199]

Selleck's RGD (Arg-Gly-Asp) Peptides has been cited by 25 publications

BMAL1/PGC1α4-FNDC5/irisin axis impacts distinct outcomes of time-of-day resistance exercise [ J Sport Health Sci, 2024, S2095-2546(24)00124-8]	PubMed: 39187065
Dendritic cells overcome Cre/Lox induced gene deficiency by siphoning cytosolic material from surrounding cells [ iScience, 2024, 27(3):109119]	PubMed: 38384841
Multimodal characterization of murine gastruloid development [ Cell Stem Cell, 2023, 30(6):867-884.e11]	PubMed: 37209681
Graphene-based 3D-Printed nanocomposite bioelectronics for monitoring breast cancer cell adhesion [ Biosens Bioelectron, 2023, 226:115113]	PubMed: 36764127
Mindin Activates Autophagy for Lipid Utilization and Facilitates White Spot Syndrome Virus Infection in Shrimp [ Mbio, 2023, Vol. 14, No. 2]	PubMed: None
Mindin Activates Autophagy for Lipid Utilization and Facilitates White Spot Syndrome Virus Infection in Shrimp [ mBio, 2023, e0291922.]	PubMed: 36779788
Structural Optimization and Interaction Study of a DNA Aptamer to L1 Cell Adhesion Molecule [ Int J Mol Sci, 2023, 24(10)8612]	PubMed: 37239955
Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae [ iScience, 2023, 26(10):107884]	PubMed: 37766995
Sandwich Culture Platforms to Investigate the Roles of Stiffness Gradients and Cell-Matrix Adhesions in Cancer Cell Migration [ Cancers (Basel), 2023, 15(6)1729]	PubMed: 36980615
Lysyl oxidase-like 4 exerts an atypical role in breast cancer progression that is dependent on the enzymatic activity that targets the cell-surface annexin A2 [ Front Oncol, 2023, 13:1142907]	PubMed: 37091157

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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