Rebaudioside A

Catalog No.S3796 Batch:S379601

Technical Data

Formula	C₄₄H₇₀O₂₃
Molecular Weight	967.01	CAS No.	58543-16-1
Solubility (25°C)*	In vitro	DMSO	40 mg/mL (41.36 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Rebaudioside A (Stevia Extract, Reb A, Glycoside A3, Stevioside A3), an natural sweetening ingredient extracted from the stevia leaf, is used in powder or liquid form to heighten sweetening intensity and improve taste. Rebaudioside A is a steviol glycoside, α-glucosidase inhibitor with IC50 of 35.01 μg/ml.can inhibit ATP-sensitive K+-channels.
In vitro	Rebaudioside A potentiates glucose-stimulated insulin secretion on MIN6 cells. It does not shange intracellular cAMP level of isolated mouse islets. Rebaudioside A modulates the effect of glucose on insulin secretion via indirect inhibition of the KATP channels, thereby allowing β cells to depolarize and generate action potentials, events that are important for glucose-stimulated insulin secretion^[1].

Description

Rebaudioside A (Stevia Extract, Reb A, Glycoside A3, Stevioside A3), an natural sweetening ingredient extracted from the stevia leaf, is used in powder or liquid form to heighten sweetening intensity and improve taste. Rebaudioside A is a steviol glycoside, α-glucosidase inhibitor with IC50 of 35.01 μg/ml.can inhibit ATP-sensitive K+-channels.

In vitro

Rebaudioside A potentiates glucose-stimulated insulin secretion on MIN6 cells. It does not shange intracellular cAMP level of isolated mouse islets. Rebaudioside A modulates the effect of glucose on insulin secretion via indirect inhibition of the KATP channels, thereby allowing β cells to depolarize and generate action potentials, events that are important for glucose-stimulated insulin secretion^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MIN6 cells Concentrations 10^-13 to 10^-7 μM Incubation Time 60 min Method After 30 min of preincubation with Krebs-Ringer bicarbonate buffer without glucose, cells are exposed to the indicated conditions for 60 min; the solution is then collected and subjected to radioimmunoassay for the detection of insulin levels.

References

[1] Abudula R, et al. Diabetes Obes Metab. 2008, 10(11):1074-85.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.