Quinidine hemisulfate hydrate

Catalog No.S3751 Batch:S375101

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Technical Data

Formula

C20H24N2O2.1/2H2SO4.H2O
Molecular Weight 391.47 CAS No. 6591-63-5
Solubility (25°C)* In vitro DMSO 100 mg/mL (255.44 mM)
Ethanol 100 mg/mL (255.44 mM)
Water 9 mg/mL (22.99 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Quinidine hemisulfate hydrate(Chinidin, Pitayine, β-quinine, (+)-quinidine) acts as a blocker of voltage-gated sodium channels, also an antimuscarinic and antimalarial.
Targets
Sodium channel [1]
In vitro

Quinidine selectively reduces the proliferation of merlin-deficient HMM cell lines by causing a G0/G1 arrest, whereas the proliferation rates of merlinexpressing HMM cell lines remain unchanged. In addtion to its function as a channel blocker, quinidine is an inhibitor of a specific isoform of the cytochrome P450 family (CYP2D6), which is involved in the detoxification and metabolism of multiple commonly prescribed drugs as well as numerous endogenous substrates[1].
In vivo

Quinidine is a potent and selective inhibitor of P450db and that quinidine can inhibit the in vivo metabolism of debrisoquine in humans. Quinidine is an inhibitor of amphetamine metabolism in vivo[2].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HMM cell lines HIB, and TRA

  • Concentrations

    2.5, 5, 10, 25 μM

  • Incubation Time

    4 days

  • Method

    Cells were cultured on T25 cm2 flasks in the presence of different concentrations of quinidine for 4 days. Bromodeoxyuridine was added for 8.5 hours, after which cells were trypsinized and spun down for 10 minutes at 1500 revolutions per minute. Cells were washed twice in 1% BSA/phosphate-buffered saline (PBS) and fixed with 70% ethanol on ice. DNA was denatured by 2N HCl/Triton X100 at room temperature for 30 minutes and sodium tetraborate was added to neutralize the pH level. After centrifugation, cells were resuspended in 1% BSA/0.5% Tween 20/PBS and the cell concentration was adjusted to 1 x 106 cells per test.
Animal Study:

[2]
  • Animal Models

    Lewis rats

  • Dosages

    80 mg/kg

  • Administration

    p.o.

Selleck's Quinidine hemisulfate hydrate has been cited by 2 publications

Pathogenesis and drug response of iPSC-derived cardiomyocytes from two Brugada syndrome patients with different Na v1.5-subunit mutations [ J Biomed Res, 2021, 35(5):395-407] PubMed: 34628405
Quantifying drug-induced structural toxicity in hepatocytes and cardiomyocytes derived from hiPSCs using a deep learning method [ J Pharmacol Toxicol Methods, 2020, 106895] PubMed: 32629158

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.