Pyrogallol

Catalog No.S3885 Batch:S388501

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Technical Data

Formula

C6H6O3
Molecular Weight 126.11 CAS No. 87-66-1
Solubility (25°C)* In vitro DMSO 25 mg/mL (198.23 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Pyrogallol (1,2,3-trihydroxybenzene), an organic compound belonging to the phenol family, used as a photographic film developer and in the preparation of other chemicals. It is known to be a superoxide anion generator and ROS inducer.
In vitro Pyrogallol (PG) inhibits the growth of human pulmonary A549 cells by inducing cell cycle arrest as well as triggering apoptosis. PG can disturb the natural oxidation and reduction equilibrium in cells. Oxidative stress by PG induces NF kappa B DNA binding and inducible NOS mRNA in cultured A549 lung epithelial cells, which may play a role in defending cells against PG-induced oxidative stress[1].
In vivo Dermal administration of pyrogallol causes increased incidences of nonneoplastic lesions of the skin at the site of application in male and female rats and mice, skin adjacent to the site of application in male and female mice, and mammary gland in female mice[2].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    A549 cells

  • Concentrations

    0-100 μM

  • Incubation Time

    72 h

  • Method

    1×106 cells are incubated with 0-100 μM of PG for 72 h. Cells are washed twice with cold PBS and then resuspended in 500 μl of binding buffer (10 mM HEPES/NaOH pH 7.4, 140 mM NaCl, 2.5 mM CaCl2) at 1×106 cells/ml. Then 5 μl of annexin V-FITC is added to these cells, which are analyzed with a FACStar flow cytometer.
Animal Study:

[2]
  • Animal Models

    Male and female F344/N rats and B6C3F1/N mice

  • Dosages

    0, 9.5, 18.75, 37.5, 75, or 150 mg/kg (rats); 0, 38, 75, 150, 300, or 600 mg/kg (mice)

  • Administration

    dermally

Selleck's Pyrogallol has been cited by 3 publications

Ginsenoside Rg1 attenuates diabetic vascular endothelial dysfunction by inhibiting the calpain-1/ROS/PKC-β axis [ Life Sci, 2023, 329:121972] PubMed: 37482213
ROS-mediated NLRP3 inflammasome activation participates in the response against Neospora caninum infection [ Parasit Vectors, 2020, 13(1):449] PubMed: 32891167
ROS-mediated NLRP3 inflammasome activation participates in the response againstNeospora caninuminfection [ Parasites & Vectors, 2020, 449-2020)] PubMed: None

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.