Puromycin aminonucleoside

Catalog No.S9631 Batch:S963101

Technical Data

Formula	C₁₂H₁₈N₆O₃
Molecular Weight	294.31	CAS No.	58-60-6
Solubility (25°C)*	In vitro	DMSO	59 mg/mL (200.46 mM)
		Water	30 mg/mL (101.93 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Puromycin aminonucleoside (NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN), the aminonucleoside portion of the antibiotic puromycin, is a reversible inhibitor of dipeptidyl-peptidase II and cytosol alanyl aminopeptidase that induces apoptosis in mesangial cells (MCs) accompanied by declined cell viability and enhanced inflammatory response.Puromycin aminonucleoside can be used to induce animal models of Kidney Disease.

Targets

DPP-2 ^[1]

cytosol alanyl aminopeptidase ^[1]

In vitro

Puromycin aminonucleoside (PAN) induces MC apoptosis accompanied by the declined cell viability and enhanced inflammatory response. In PAN-treated MCs, ERRα overexpression further aggravates PAN-induced apoptosis.^[1]

In vivo

In agreement with the in vitro study, increased ERRα expression is observed in line with enhanced apoptotic response in renal cortex from PAN-treated rats.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines mouse mesangial cells Concentrations 50 μg/ml, 100 μg/ml Incubation Time 24 h Method For apoptosis assay, mesangial cells are treated with PAN for 24 h at the indicated concentration. After treatment, cells are stained with FITC-Annexin V and PI according to the manufacturer’s protocol. Cell apoptosis is determined using a BD FACS Calibur flow cytometer and data is analyzed with Flowjo software.
Animal Study:^{^[1]}	Animal Models male Sprague-Dawley rats Dosages 150 mg/kg Administration IV

Cell Assay:^{^[1]}

Cell lines
mouse mesangial cells
Concentrations
50 μg/ml, 100 μg/ml
Incubation Time
24 h
Method
For apoptosis assay, mesangial cells are treated with PAN for 24 h at the indicated concentration. After treatment, cells are stained with FITC-Annexin V and PI according to the manufacturer’s protocol. Cell apoptosis is determined using a BD FACS Calibur flow cytometer and data is analyzed with Flowjo software.

Animal Study:^{^[1]}

Animal Models
male Sprague-Dawley rats
Dosages
150 mg/kg
Administration
IV

References

[1] Wei Gong, et al. Am J Physiol Renal Physiol. 2019 May 1;316(5):F906-F913.

Selleck's Puromycin aminonucleoside has been cited by 5 publications

Suppression of ZBP1-mediated NLRP3 inflammasome by the tegument protein VP22 facilitates pseudorabies virus infection [ mBio, 2024, 15(12):e0194524]	PubMed: 39475237
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62]	PubMed: 37525118
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62]	PubMed: 37525118
SNAI2 promotes the malignant transformation of oral leukoplakia by modulating p-EMT [ Oral Dis, 2022, 10.1111/odi.14321]	PubMed: 35894087
PD-L1 confers glioblastoma multiforme malignancy via Ras binding and Ras/Erk/EMT activation [ Biochim Biophys Acta Mol Basis Dis, 2018, 1864(5 Pt A):1754-1769]	PubMed: 29510196

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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