Puromycin

Catalog No.E4996 Batch:E499601

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Technical Data

Formula

C22H29N7O5
Molecular Weight 471.51 CAS No. 53-79-2
Solubility (25°C)* In vitro DMSO 94 mg/mL (199.35 mM)
Ethanol 4 mg/mL (8.48 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.45mg/ml Taking the 1 mL working solution as an example, add 50 μL of 9 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.7mg/ml Taking the 1 mL working solution as an example, add 50 μL of 94 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Puromycin (CL13900, Puromycine) is an antibiotic, analog of the 3' end of aminoacyl-tRNA and an inhibitor of protein synthesis that mimics tyrosyl-tRNA, causing premature termination of translation. It blocks translation by labeling and releasing elongating polypeptide chains from ribosomes and has been widely used in molecular biology research.

Protocol (from reference)

Selleck's Puromycin has been cited by 12 publications

Alterations in PD-L1 succinylation shape anti-tumor immune responses in melanoma [ Nat Genet, 2025, 57(3):680-693] PubMed: 40069506
BRD8 Guards the Pluripotent State by Sensing and Maintaining Histone Acetylation [ Adv Sci (Weinh), 2025, 12(5):e2409160] PubMed: 39656858
A novel pathway for stemness propagation and chemoresistance in non-small cell lung cancer via phosphorylated PKM2-loaded small extracellular vesicles [ Theranostics, 2025, 15(8):3439-3461] PubMed: 40093893
N6-methyladenosine in 28S rRNA promotes oncogenic mRNA translation and tyrosine catabolism [ Cell Rep, 2025, 44(1):115139] PubMed: 39739529
PTOV1 exerts pro-oncogenic role in colorectal cancer by modulating SQSTM1-mediated autophagic degradation of p53 [ J Transl Med, 2025, 23(1):157] PubMed: 39905441
DLAT is involved in ovarian cancer progression by modulating lipid metabolism through the JAK2/STAT5A/SREBP1 signaling pathway [ Cancer Cell Int, 2025, 25(1):25] PubMed: 39871246
Integrative analysis of single-cell and transcriptome sequencing with experimental validation reveals PKHD1L1 as a novel biomarker in lung adenocarcinoma [ Sci Rep, 2025, 15(1):2795] PubMed: 39843484
METTL3 stabilizes SERPINE2 via the m6A modification to drive the malignant progression of gastric signet ring cell carcinoma [ Transl Cancer Res, 2025, 14(1):78-92] PubMed: 39974426
UPF3B modulates endoplasmic reticulum stress through interaction with inositol-requiring enzyme-1α [ Cell Death Dis, 2024, 15(8):587] PubMed: 39138189
Mitochondrial transplantation rescues Ca2+ homeostasis imbalance and myocardial hypertrophy in SLC25A3-related hypertrophic cardiomyopathy [ Cell Rep, 2024, 43(12):115065] PubMed: 39671292

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.