PTP Inhibitor I

Catalog No.S3696 Batch:S369601

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Technical Data

Formula

C8H7BrO2
Molecular Weight 215.04 CAS No. 2491-38-5
Solubility (25°C)* In vitro DMSO 43 mg/mL (199.96 mM)
Ethanol 43 mg/mL (199.96 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description PTP Inhibitor I is a cell-permeable, protein tyrosine phosphatase (PTP) inhibitor with Ki values of 43 and 42 μM for SHP-1(ΔSH2) and PTP1B, respectively.
Targets
PTP1B [1]
(Cell-free assay)		 SHP-1(ΔSH2) [1]
(Cell-free assay)
42 μM(Ki) 43 μM(Ki)
In vitro PTP Inhibitor I binds to PTPs with the highest affinity, having Ki values of 43 and 42 μM and Kinact values of 0.40 and 0.57/min for SHP-1(△SH2) and PTP1B, respectively. It has lower affinity to wild-type SHP-1 than its catalytic domain (K= 530 vs 43 μM), likely due to the fact that the SH2 domains can directly bind to the PTP active site and interfere with substrate/inhibitor binding[1].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    human B cells

  • Concentrations

    30, 100, 300, 1000 μM

  • Incubation Time

    3 mins

  • Method

    After incubation with varying concentrations of PTP Inhibitor I for 3 min, the cells are lysed, and the cellular proteins are separated on an SDS-PAGE gel, followed by western blot analysis.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.