Psoralen

Catalog No.S4737 Batch:S473701

Technical Data

Formula	C₁₁H₆O₃
Molecular Weight	186.16	CAS No.	66-97-7
Solubility (25°C)*	In vitro	DMSO	37 mg/mL (198.75 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Psoralen (Psoralene, Ficusin, Furocoumarin) is a naturally occurring furocoumarin that intercalates with DNA, inhibiting DNA synthesis and cell division.
In vitro	Psoralen inhibits the proliferation of MCF-7/ADR cells as shown by G0/G1 phase arrest rather than encouraging apoptosis. Psoralen reverses MDR(multidrug resistance) through inhibiting ATPase activity rather than reducing P-gp expression. Psoralen inhibits the migration abilities of MCF-7/ADR cells by repressing EMT possibly through inhibiting the activation of NF-κB. Psoralens are photoactive compounds that readily alkylate DNA when activated by longwave ultraviolet light. Proliferation has been significantly promoted in MCF-7/ADR cells treated with low concentration of psoralen (<10.75 µM) and inhibited with high concentration(>21.5 µM). Psoralen can inhibit metastasis of breast cancer. Psoralen mediates a variety of cell processes including cell death, proliferation, inflammation and migration^[1].
In vivo	Psoralen has been characterized as a tumor suppressor in various tumors^[1]. Psoralen ameliorates sex hormone deficiency-induced osteoporosis in female and male mice. It has antiosteoporosis effect in ovariectomy-induced osteoporotic rats via stimulating osteoblastic differentiation from bone mesenchymal stem cells^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human breast cancer MCF-7/ADR cells, MCF-10A, and MCF-7 cells Concentrations 0, 21.5, 43.0, 64.5, 86.0, 107.5 µM Incubation Time 48 h Method The effects of psoralen on cell proliferation are measured by MTT assay. MCF-10A and MCF-7/ADR cells are cultured in 96-well plates at a cell density of 2×10⁴ cells per well for 48 h. The medium is then removed and replaced by fresh medium containing different concentrations of psoralen (0, 21.5, 43.0, 64.5, 86.0, 107.5 μM) for 48 h. Cells in the negative control group are incubated with RPMI-1640 culture medium supplemented with 0.1% dimethyl sulfoxide (DMSO). Cells are incubated with 10 µL MTT (5 mg/mL) for 4 h, and then discarded the medium and added 200 µL DMSO. The spectrophotometric absorbance is measured at 490 nm with enzyme-labeling instrument after the crystals were fully dissolved.
Animal Study:^{^[2]}	Animal Models ICR mice Dosages 10 mg/kg and 20 mg/kg Administration intragastrically

Cell Assay:^{^[1]}

Cell lines
Human breast cancer MCF-7/ADR cells, MCF-10A, and MCF-7 cells
Concentrations
0, 21.5, 43.0, 64.5, 86.0, 107.5 µM
Incubation Time
48 h
Method
The effects of psoralen on cell proliferation are measured by MTT assay. MCF-10A and MCF-7/ADR cells are cultured in 96-well plates at a cell density of 2×10⁴ cells per well for 48 h. The medium is then removed and replaced by fresh medium containing different concentrations of psoralen (0, 21.5, 43.0, 64.5, 86.0, 107.5 μM) for 48 h. Cells in the negative control group are incubated with RPMI-1640 culture medium supplemented with 0.1% dimethyl sulfoxide (DMSO). Cells are incubated with 10 µL MTT (5 mg/mL) for 4 h, and then discarded the medium and added 200 µL DMSO. The spectrophotometric absorbance is measured at 490 nm with enzyme-labeling instrument after the crystals were fully dissolved.

Animal Study:^{^[2]}

Animal Models
ICR mice
Dosages
10 mg/kg and 20 mg/kg
Administration
intragastrically

References

Selleck's Psoralen has been cited by 1 publication

Coumarins of Lovage Roots (Levisticum officinale W.D.J.Koch): LC-MS Profile, Quantification, and Stability during Postharvest Storage [ Metabolites, 2022, 13(1)3]	PubMed: 36676932

Coumarins of Lovage Roots (Levisticum officinale W.D.J.Koch): LC-MS Profile, Quantification, and Stability during Postharvest Storage [ Metabolites, 2022, 13(1)3]

PubMed: 36676932

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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