Procyanidin C1

Catalog No.E0478 Batch:E047802

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Technical Data

Formula

C45H38O18
Molecular Weight 866.77 CAS No. 37064-30-5
Solubility (25°C)* In vitro DMSO 100 mg/mL (115.37 mM)
 
 
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Procyanidin C1 is a polyphenolic component of grape seed extract, increases the healthspan and lifespan of mice through its action on senescent cells. PCC1 is a natural senotherapeutic agent with in vivo activity and a potential intervention to delay, alleviate or prevent age-related pathologies.
In vitro

Procyanidin C1 induces DNA damage, cell cycle arrest and enhanced the expression of checkpoint kinases. Procyanidin C1 decreases the level of Bcl-2 but increases the BAX, caspase 3 and 9 expression in cancer cells.[1]
In vivo

In rodent models, Procyanidin C1 depletes senescent cells in a treatment-damaged tumour microenvironment and enhances therapeutic efficacy when co-administered with chemotherapy. Intermittent administration of Procyanidin C1 to either irradiated, senescent cell-implanted or naturally aged old mice alleviates physical dysfunction and prolongs survival.[2]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    MDA-MB- 231 cell line, MCF-7 cell line

  • Concentrations

    48 h

  • Incubation Time

    6.25-100 mg/ml

  • Method

    Overnight grown cells (5×103cells/well) are treated for 48 h with procyanidin C1 at a concentration ranging from 6.25 to 100 mg/ml in final volume of 150 ml. Cells without treatment served as control. After treatment, cells are used for MTT assay and cell cycle phase distribution analysis. Analysis of Bcl-2 or BAX levels is performed using an ELISA method.
Animal Study:

[2]
  • Animal Models

    C57BL/6J mice (males, 8–12 weeks)

  • Dosages

    20 mg/kg

  • Administration

    i.p.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.