Procyanidin C1

Catalog No.E0478 Batch:E047801

Technical Data

Formula	C₄₅H₃₈O₁₈
Molecular Weight	866.77	CAS No.	37064-30-5
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (115.37 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Procyanidin C1 is a polyphenolic component of grape seed extract, increases the healthspan and lifespan of mice through its action on senescent cells. PCC1 is a natural senotherapeutic agent with in vivo activity and a potential intervention to delay, alleviate or prevent age-related pathologies.
In vitro	Procyanidin C1 induces DNA damage, cell cycle arrest and enhanced the expression of checkpoint kinases. Procyanidin C1 decreases the level of Bcl-2 but increases the BAX, caspase 3 and 9 expression in cancer cells.^[1]
In vivo	In rodent models, Procyanidin C1 depletes senescent cells in a treatment-damaged tumour microenvironment and enhances therapeutic efficacy when co-administered with chemotherapy. Intermittent administration of Procyanidin C1 to either irradiated, senescent cell-implanted or naturally aged old mice alleviates physical dysfunction and prolongs survival.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MDA-MB- 231 cell line, MCF-7 cell line Concentrations 48 h Incubation Time 6.25-100 mg/ml Method Overnight grown cells (5×10³cells/well) are treated for 48 h with procyanidin C1 at a concentration ranging from 6.25 to 100 mg/ml in final volume of 150 ml. Cells without treatment served as control. After treatment, cells are used for MTT assay and cell cycle phase distribution analysis. Analysis of Bcl-2 or BAX levels is performed using an ELISA method.
Animal Study:^{^[2]}	Animal Models C57BL/6J mice (males, 8–12 weeks) Dosages 20 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
MDA-MB- 231 cell line, MCF-7 cell line
Concentrations
48 h
Incubation Time
6.25-100 mg/ml
Method
Overnight grown cells (5×10³cells/well) are treated for 48 h with procyanidin C1 at a concentration ranging from 6.25 to 100 mg/ml in final volume of 150 ml. Cells without treatment served as control. After treatment, cells are used for MTT assay and cell cycle phase distribution analysis. Analysis of Bcl-2 or BAX levels is performed using an ELISA method.

Animal Study:^{^[2]}

Animal Models
C57BL/6J mice (males, 8–12 weeks)
Dosages
20 mg/kg
Administration
i.p.

References

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.