Poloxin

Catalog No.S5893 Batch:S589301

Technical Data

Formula	C₁₈H₁₉NO₃
Molecular Weight	297.35	CAS No.	321688-88-4
Solubility (25°C)*	In vitro	DMSO	59 mg/mL (198.41 mM)
		Ethanol	30 mg/mL (100.89 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Poloxin is a non-ATP competitive Polo-like Kinase 1 polo-box domain (Plk1 PBD) inhibitor with an apparent IC50 of 4.8 μM. Poloxin's IC50 values against the PBDs of Plk2 and Plk3 as Plk1 are approximately 4-fold and 11-fold higher, respectively in fluorescence polarization assays.

Targets

Plk1 PBD ^[1]
(in fluorescence polarization assays)

4.8 μM

In vitro

Poloxin is a small-molecule inhibitor specifically targeting the function of the polo-box domain (PBD) of Polo-like kinase 1 (Plk1), which strongly inhibits proliferation of cancer cells by inducing mitotic arrest, followed by a surge of apoptosis.^[2]

In vivo

Poloxin significantly suppresses tumor growth of cancer cell lines in xenograft mouse models by lowering the proliferation rate and triggering apoptosis in treated tumor tissues.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HeLa cells Concentrations 25 µM Incubation Time 10 h Method HeLa cells are synchronized with double thymidine block and released into fresh medium with either DMSO or 25 µM Poloxin for 10 hours. Cells are fixed and stained for α-tubulin, pericentrin, and DNA. The morphological features of centrosomes, spindles, and chromosomes are examined by fluorescence microscopy.

Cell Assay:^{^[2]}

Cell lines
HeLa cells
Concentrations
25 µM
Incubation Time
10 h
Method
HeLa cells are synchronized with double thymidine block and released into fresh medium with either DMSO or 25 µM Poloxin for 10 hours. Cells are fixed and stained for α-tubulin, pericentrin, and DNA. The morphological features of centrosomes, spindles, and chromosomes are examined by fluorescence microscopy.

References

Selleck's Poloxin has been cited by 1 publication

Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry [ Nat Commun, 2023, 14(1):355]	PubMed: 36683055

Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry [ Nat Commun, 2023, 14(1):355]

PubMed: 36683055

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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