Pluripotin (SC1)

Catalog No.S7752 Batch:S775201

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Technical Data

Formula

C27H25F3N8O2
Molecular Weight 550.53 CAS No. 839707-37-8
Solubility (25°C)* In vitro DMSO 100 mg/mL (181.64 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
0.83mg/ml Taking the 1 mL working solution as an example, add 50 μL of 16.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Pluripotin (SC1) is a dual inhibitor of extracellular signal-regulated kinase 1 (ERK1, MAPK3) and RasGAP. Maintains embryonic stem cell (ESC) self-renewal. Pluripotin also inhibits RSK1, RSK2, RSK3 and RSK4 with IC50 of 0.5 µM, 2.5 µM, 3.3 µM and 10.0 µM, respectively.
Targets
RSK1 [3] ERK1 [1]
(Cell-free assay)		 RasGAP [1]
(Cell-free assay)		 RSK1 [3]
(Cell-free assay)		 RSK2 [3]
(Cell-free assay)		 View More
98 nM(Kd) 212 nM(Kd) 0.5 μM 2.5 μM
In vitro

SC1 is a dual function small molecule inhibitor of both ERK1 and RasGAP and that simultaneous inhibition of both protein activities is required and sufficient for SC1’s effects on mES cells. SC1 activates Ras by inhibition of RasGAP function[1]. SC1 improved the derivation efficiency and pluripotency of ES cells from C57BL/6. Three types of pluripotent stem cells (fES, ntES, and iPS cells) of the C57BL/6 background could generate full-term pups with high efficiency when cultured with SC1[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Mouse ES cells

  • Concentrations

    300 nM, 1 μM, 3 μM

  • Incubation Time

    --

  • Method

    mES cells are seeded in gelatin-coated six-well plates at 1.6×104 cells/cm2 and maintained with 3 μM SC1 in ESC-SR media, 1 μM SC1 in ESC-N2B27 media, or 300 nM SC1 in ESC-N2 media without LIF or feeder cells. mES cells maintained with 103 units/ml LIF plus 10 ng/ml BMP4 in ESC-N2B27 media are used as a positive control. mES cells treated with DMSO in ESC-SR media, ESC-N2B27 media, or ESC-N2 media for two passages are used as negative controls. Cells are split every 3 days and seeded at same density (1.6×104 cells/cm2). mES cells at passage 11 are analyzed with FACS, immunocytochemistry, histocytochemistry, and RT-PCR.

Selleck's Pluripotin (SC1) has been cited by 3 publications

Slowly progressive cell death induced by GPx4-deficiency occurs via MEK1/ERK2 activation as a downstream signal after iron-independent lipid peroxidation [ J Clin Biochem Nutr, 2024, 74(2):97-107] PubMed: 38510679
Repurposed FDA-Approved drug sorafenib reduces replication of Venezuelan equine encephalitis virus and other alphaviruses [Lundberg L Antiviral Res, 2018, 157:57-67] PubMed: 29981794
SC1 sustains the self-renewal capacity and pluripotency of chicken blastodermal cells by inhibiting the phosphorylation of ERK1 and promoting the phosphorylation of Akt. [ Reprod Domest Anim, 2018, 53(5):1052-1059] PubMed: 30028046

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.