Pimonidazole

Catalog No.S9919 Batch:S991901

Technical Data

Formula	C₁₁H₁₈N₄O₃
Molecular Weight	254.29	CAS No.	70132-50-2
Solubility (25°C)*	In vitro	DMSO	51 mg/mL (200.55 mM)
		Ethanol	51 mg/mL (200.55 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Pimonidazole (Ro 03-8799) is a novel hypoxia marker used for complementary study of tumor hypoxia and cell proliferation in cervical carcinoma.
In vitro	Pimonidazole has radio-sensitivity and recovery capacity from radiation-induced damage depend on p53 status of the tumor cell.<sup><a class="sref" href="#s_ref">[3]</a></sup>
In vivo	Pimonidazole can overcome radiation resistance in hypoxiaby melanoma cells, which is enhanced by liposome modification.<sup><a class="sref" href="#s_ref">[2]</a></sup>

Protocol (from reference)

Cell Assay:^{<sup><a class="sref" href="#s_ref">[3]</a></sup>}	Cell lines HMV-II human malignant melanoma cells Concentrations 0.5 mM Incubation Time 1–4 h Method HMV-II cells are seeded by 2,000 cells per well in 96-well culture plates, allowed to adhere for 30 hr in a humidified atmosphere with 5% CO<sub>2</sub> at 37 C, and followed by incubation in a hypoxic condition of 100% N2 set in a glass desicator for 18 hr. The liposomal Pmz or free-Pmz is applied in 100 μL of degassed medium and incubated for 3 hr in a hypoxic condition of 100% N2 set in a glass desicator. After X-ray irradiation, the cells are incubated for 3 hr in a humidified atmosphere with 5% CO2 at 37 C. The cells are confirmed to survive according to either morphological observation or cell viability assay using the redox indicator WST-8.
Animal Study:^{<sup><a class="sref" href="#s_ref">[2]</a></sup>}	Animal Models Human head and neck cancer xenograft model mice (BALB/c athymic nude mice; inoculated with FaDu cells) Dosages 200 mg/kg Administration IOCV

Cell Assay:^{<a class="sref" href="#s_ref">[3]</a>}

Cell lines
HMV-II human malignant melanoma cells
Concentrations
0.5 mM
Incubation Time
1–4 h
Method
HMV-II cells are seeded by 2,000 cells per well in 96-well culture plates, allowed to adhere for 30 hr in a humidified atmosphere with 5% CO2 at 37 C, and followed by incubation in a hypoxic condition of 100% N2 set in a glass desicator for 18 hr. The liposomal Pmz or free-Pmz is applied in 100 μL of degassed medium and incubated for 3 hr in a hypoxic condition of 100% N2 set in a glass desicator. After X-ray irradiation, the cells are incubated for 3 hr in a humidified atmosphere with 5% CO2 at 37 C. The cells are confirmed to survive according to either morphological observation or cell viability assay using the redox indicator WST-8.

Animal Study:^{<a class="sref" href="#s_ref">[2]</a>}

Animal Models
Human head and neck cancer xenograft model mice (BALB/c athymic nude mice; inoculated with FaDu cells)
Dosages
200 mg/kg
Administration
IOCV

References

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SHIPPING AND STORAGE
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