Pilocarpine HCl

Catalog No.S4231 Batch:S423111

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Technical Data

Formula

C11H16N2O2.HCl
Molecular Weight 244.72 CAS No. 54-71-7
Solubility (25°C)* In vitro DMSO 49 mg/mL (200.22 mM)
Water 49 mg/mL (200.22 mM)
Ethanol 49 mg/mL (200.22 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.4mg/ml Taking the 1 mL working solution as an example, add 50 μL of 8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.45mg/ml Taking the 1 mL working solution as an example, add 50 μL of 49 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Pilocarpine HCl (NSC 5746) is a nonselective muscarinic acetylcholine receptor agonist used to produce an experimental model of epilepsy.
Targets
mAChR [1]
In vitro Pilocarpine is a parasympathomimetic alkaloid obtained from the leaves of tropical American shrubs from the genus Pilocarpus. It is a non-selective muscarinic receptor agonist in the parasympathetic nervous system, which acts therapeutically at themuscarinic acetylcholine receptor M3 due to its topical application, e.g., in glaucoma and xerostomia. Pilocarpine acts on a subtype of muscarinic receptor (M3) found on the iris sphincter muscle, causing the muscle to contract and engage in miosis. [1] Pilocarpine also acts on the ciliary muscle and causes it to contract. When the ciliary muscle contracts, it opens the trabecular meshwork through increased tension on the scleral spur. This action facilitates the rate that aqueous humorleaves the eye to decrease intraocular pressure. In ophthalmology, pilocarpine is also used to reduce the possibility of glare at night from lights when the patient has undergone implantation of phakic intraocular lenses; the use of pilocarpine would reduce the size of the pupils, relieving these symptoms. The most common concentration for this use is pilocarpine 1%, the weakest concentration. Pilocarpine is also used to treat dry mouth (xerostomia) which can occur, for example, as a side effect of radiation therapy for head and neck cancers. Pilocarpine stimulates the secretion of large amounts of saliva and sweat.[2]

Protocol (from reference)

Selleck's Pilocarpine HCl has been cited by 9 publications

GPCR Inhibitors Have Antiviral Properties against JC Polyomavirus Infection [ Viruses, 2024, 16(10)1559] PubMed: 39459893
SREBP1 deficiency diminishes glutamate-mediated HT22 cell damage and hippocampal neuronal pyroptosis induced by status epilepticus [ Heliyon, 2024, 10(1):e23945] PubMed: 38205297
Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage [ Theranostics, 2021, 11(12):5986-6005] PubMed: 33897894
Decreased expression of the clock gene Bmal1 is involved in the pathogenesis of temporal lobe epilepsy [ Mol Brain, 2021, 14(1):113] PubMed: 34261484
A Low Dose of Aripiprazole Has the Strongest Sensitization Effect Among 19 Repositioned Bipolar Drugs in P-gp-overexpressing Drug-resistant Cancer Cells [ Anticancer Res, 2021, 41(2):687-697] PubMed: 33517273
Knockdown of ZFAS1 Inhibits Hippocampal Neurons Apoptosis and Autophagy by Activating the PI3K/AKT Pathway via Up-regulating miR-421 in Epilepsy [ Neurochem Res, 2020, 10.1007/s11064-020-03103-1] PubMed: 32725369
Phosphorylation of PRAS40 contributes to the activation of the PI3K/AKT/mTOR signaling pathway and the inhibition of autophagy following status epilepticus in rats [ Exp Ther Med, 2020, 20(4):3625-3632] PubMed: 32855714
Conformational Complexity and Dynamics in a Muscarinic Receptor Revealed by NMR Spectroscopy. [ Mol Cell, 2019, 75(1):53-65] PubMed: 31103421
WDR45 contributes to neurodegeneration through regulation of ER homeostasis and neuronal death. [ Autophagy, 2019, 10.1080/15548627.2019.1630224] PubMed: 31204559

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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