Picroside I

Catalog No.S3787 Batch:S378701

Technical Data

Formula	C₂₄H₂₈O₁₁
Molecular Weight	492.47	CAS No.	27409-30-9
Solubility (25°C)*	In vitro	DMSO	98 mg/mL (198.99 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Picroside I (6'-Cinnamoylcatalpol), an iridoid glycoside, is a hepatoprotective agent which is reported to be antimicrobial and used against hepatitis B.
In vitro	Picrosides I and II cause a concentration-dependent (> 0.1 μM) enhancement of basic fibroblast growth factor (bFGF, 2 ng/ml)-, staurosporine (10 nM)- and dibutyryl cyclic AMP (dbcAMP, 0.3 mM)-induced neurite outgrowth from PC12D cells. Picrosides activate MAP kinase-dependent signaling pathway^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines PC12D cells Concentrations 60 μM Incubation Time 10 min Method For Western blot analysis, PC12D cells are plated at a cell density of 1×10⁶ cells/35-mm culture dish, and cultured in the culture medium overnight. Cells are treated with or without picroside I or II 60 μM in the presence or absence of 7S NGF for 10 min. After the cells are washed with ice-cold PBS, they are lysed. Cell lysates are boiled for 5 min and subsequently sonicated for 3 min. Cell lysates are separated by SDS-polyacrylamide gel electrophoresis PAGE and transferred onto polyvinylidene difluoride membranes.

Cell Assay:^{^[1]}

Cell lines
PC12D cells
Concentrations
60 μM
Incubation Time
10 min
Method
For Western blot analysis, PC12D cells are plated at a cell density of 1×10⁶ cells/35-mm culture dish, and cultured in the culture medium overnight. Cells are treated with or without picroside I or II 60 μM in the presence or absence of 7S NGF for 10 min. After the cells are washed with ice-cold PBS, they are lysed. Cell lysates are boiled for 5 min and subsequently sonicated for 3 min. Cell lysates are separated by SDS-polyacrylamide gel electrophoresis PAGE and transferred onto polyvinylidene difluoride membranes.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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