Phytohemagglutinin (PHA)

Catalog No.S6990 Batch:S699001

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Technical Data

Formula

N/A
Molecular Weight N/A CAS No. 9008-97-3
Solubility (25°C)* In vitro Water 100 mg/mL
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Phytohemagglutinin (PHA) is expressed in Pichia pastoris using native signal peptides, or the Saccharomyces alpha-factor preprosequence, to direct proteins into the secretory pathway. Phytohemagglutinin induces apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3.
Targets
Bax [2] caspases-3 [2]
In vitro

Stimulation of human mononuclear leukocytes (MNL) by phytohemagglutinin induces the expression of ChAT mRNA, and potentiated ACh synthesis. Phytohemagglutinin binds to the membranes of T-cells, stimulates metabolic activity, cell division, and involves inflammatory pathways. [3]
In vivo

Phytohemagglutinin, a standard T cell mitogen, can inhibit both T- and B-cell tumors. Phytohemagglutinin (100 μg; i.p.; daily for 2 days) inhibits A20 tumor growth in CB17-SCID mice.[4]

Protocol (from reference)

Cell Assay:[3]
  • Cell lines

    Human mononuclear leukocytes

  • Concentrations

    5 μg/ml

  • Incubation Time

    0-72 h

  • Method

    MNL cells are cultured for 0-72 h in a culture dish with RPMI 1640 medium containing 10% FCS in the presence or absence of PHA. For the studies on ACh content and release of ACh into the culture medium, MNL cells are cultured for a further 24 h in RPMI 1640 medium containing 1 mM DFP (a ChE inhibitor). The content of ACh and its release into the culture medium are determined using a specific, sensitive RIA.

Selleck's Phytohemagglutinin (PHA) has been cited by 1 publication

Selenium Nanoparticles Based on Morinda officinalis Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro [ Molecules, 2023, 28(6)2426] PubMed: 36985397

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.