PMA (Phorbol 12-myristate 13-acetate)

Catalog No.S7791 Batch:S779102

Technical Data

Formula	C₃₆H₅₆O₈
Molecular Weight	616.83	CAS No.	16561-29-8
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (162.11 mM)
		Ethanol	100 mg/mL (162.11 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	PMA (Phorbol 12-myristate 13-acetate), a potent activator of PKC, is active at nanomolar concentrations. Phorbol 12-myristate 13-acetate (PMA) induces sphingosine-1-phosphate (S1P).PMA has strong excitability on the skin and mucous membrane. Special protection is required when using PMA. Wear gloves and mask to avoid direct contact in any way.
In vitro	In endothelial cells, PMA increases levels of Thy-1 mRNA and protein, induces Thy-1 up-regulation and inhibits capillary-like tube formation and endothelial cell migration.^[1]
In vivo	In the zebrafish model, PMA initially enhances endothelial cell migration, subsequently activates the PKC-δ/Syk/NF-κB-mediated pathway to up-regulate Thy-1, which in turn inhibits endothelial cell migration.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human umbilical vein endothelial cells (HUVECs), human dermal microvascular endothelial cells (HDMECs), human pulmonary microvascular endothelial cell line (HPMECs) Concentrations 20 ng/mL Incubation Time 14, 16 and 18 h for levels of Ty-1 mRNA, 20, 24 and 28 h for levels of Ty-1 protein Method After transfection with Ty-1 promoter plasmid (0.5 μg) and Renilla plasmid (50ng), the cells are treated with PMA (20ng/mL), and washed with ice-cold phosphate-buffered saline (PBS), and then lysed in passive lysis buffer. Luciferase activity is measured using the Dual-Luciferase Reporter Assay kit.
Animal Study:^{^[1]}	Animal Models Transgenic Tg(fi1:EGFP)y1 embryos of zebrafsh (Danio rerio) Dosages 20 ng/mL Administration --

Cell Assay:^{^[1]}

Cell lines
Human umbilical vein endothelial cells (HUVECs), human dermal microvascular endothelial cells (HDMECs), human pulmonary microvascular endothelial cell line (HPMECs)
Concentrations
20 ng/mL
Incubation Time
14, 16 and 18 h for levels of Ty-1 mRNA, 20, 24 and 28 h for levels of Ty-1 protein
Method
After transfection with Ty-1 promoter plasmid (0.5 μg) and Renilla plasmid (50ng), the cells are treated with PMA (20ng/mL), and washed with ice-cold phosphate-buffered saline (PBS), and then lysed in passive lysis buffer. Luciferase activity is measured using the Dual-Luciferase Reporter Assay kit.

Animal Study:^{^[1]}

Animal Models
Transgenic Tg(fi1:EGFP)y1 embryos of zebrafsh (Danio rerio)
Dosages
20 ng/mL
Administration
--

References

[1] Wen HC, et al. Sci Rep. 2018 Nov 2;8(1):16247.

Selleck's PMA (Phorbol 12-myristate 13-acetate) has been cited by 60 publications

Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for CD8+ T cell exhaustion and anti-PD-1 resistance [ Cell Rep Med, 2024, 5(8):101686]	PubMed: 39168104
GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression [ J Exp Clin Cancer Res, 2024, 43(1):105]	PubMed: 38576043
LINC00330/CCL2 axis-mediated ESCC TAM reprogramming affects tumor progression [ Cell Mol Biol Lett, 2024, 29(1):77]	PubMed: 38769475
CircMLH3 induces mononuclear macrophage pyroptosis in sepsis by sponging miR-590-3p to regulate TAK1 expression [ Int J Biol Macromol, 2024, 263(Pt 1):130179]	PubMed: 38378118
ALIX and TSG101 are essential for cellular entry and replication of two porcine alphacoronaviruses [ PLoS Pathog, 2024, 20(3):e1012103]	PubMed: 38489378
Palmitoylation of NLRP3 Modulates Inflammasome Activation and Inflammatory Bowel Disease Development [ J Immunol, 2024, ji2300241]	PubMed: 38949555
Gastric cancer cell-derived exosomal miR-541-5p induces M2 macrophage polarization through DUSP3/JAK2/STAT3 pathway [ BMC Cancer, 2024, 24(1):957]	PubMed: 39103776
AP-1 Mediates Cellular Adaptation and Memory Formation During Therapy Resistance [ bioRxiv, 2024, 2024.07.25.604999]	PubMed: 39091739
Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer [ Cancer Commun (Lond), 2023, 43(8):909-937]	PubMed: 37434399
Targeting TCF19 sensitizes MSI endometrial cancer to anti-PD-1 therapy by alleviating CD8+ T cell exhaustion via TRIM14-IFN-β axis [ Cell Rep, 2023, 42(8):112944]	PubMed: 37566545

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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