PF-431396

Catalog No.S7644 Batch:S764401

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Technical Data

Formula

C22H21F3N6O3S
Molecular Weight 506.5 CAS No. 717906-29-1
Solubility (25°C)* In vitro DMSO 100 mg/mL (197.43 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description PF-431396 is a dual PYK2/FAK inhibitor with IC50 of 11 nM and 2 nM, respectively.
Targets
FAK [1] PYK2 [1]
2 nM 11 nM
In vitro In A20 cells, PF-431396 blocks anti-Ig- and clustering LFA-1-induced tyrosine phosphorylation of Pyk2 and FAK, and further blocks B cell spreading. [2] PF-431396 consistently inhibits the increase in protein tyrosine phosphorylation (PY) induced by the absence of added calcium and induced by W-7 in the presence of calcium. [3]

Protocol (from reference)

Selleck's PF-431396 has been cited by 12 publications

Integrin signaling is critical for myeloid-mediated support of T-cell acute lymphoblastic leukemia [ Nat Commun, 2023, 14(1):6270] PubMed: 37805579
Integrin signaling is critical for myeloid-mediated support of T-cell acute lymphoblastic leukemia [ Nat Commun, 2023, 14(1):6270] PubMed: 37805579
Architecture of androgen receptor pathways amplifying glucagon-like peptide-1 insulinotropic action in male pancreatic β cells [ Cell Rep, 2023, 42(5):112529] PubMed: 37200193
Focal adhesion kinase inhibitor TAE226 combined with Sorafenib slows down hepatocellular carcinoma by multiple epigenetic effects [ J Exp Clin Cancer Res, 2021, 40(1):364] PubMed: 34784956
SHED-Dependent Oncogenic Signaling of the PEAK3 Pseudo-Kinase [ Cancers (Basel), 2021, 13(24)6344] PubMed: 34944965
A FAK/HDAC5 signaling axis controls osteocyte mechanotransduction [ Nat Commun, 2020, 11(1):3282] PubMed: 32612176
Myeloid cells provide critical support for T-ALL in vivo [ The University of Texas at Austin, 2020, ] PubMed: None
Inositol polyphosphate multikinase is a metformin target that regulates cell migration [ FASEB J, 2019, 33(12):14137-14146] PubMed: 31657647
Focal adhesion kinase a potential therapeutic target for pancreatic cancer and malignant pleural mesothelioma. [ Cancer Biol Ther, 2018, 19(4):316-327] PubMed: 29303405
Focal Adhesion- and IGF1R-Dependent Survival and Migratory Pathways Mediate Tumor Resistance to mTORC1/2 Inhibition. [ Mol Cell, 2017, 67(3):512-527] PubMed: 28757207

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.