Pepstatin A

Catalog No.S7381 Batch:S738112

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Technical Data

Formula

C34H63N5O9
Molecular Weight 685.89 CAS No. 26305-03-3
Solubility (25°C)* In vitro DMSO 60 mg/mL (87.47 mM)
Ethanol 2 mg/mL (2.91 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Pepstatin A (Pepstatin) is a potent aspartic protease inhibitor, and also inhibits HIV replication. Pepstatin A is also an inhibitor of cathepsins D and cathepsins E. Pepstatin A inhibits autophagy by suppressing lysosomal proteases.
Targets
aspartic protease [1] cathepsins D [2] cathepsins E [2]
In vitro

Pepstatin A shows effective inhibition on aspartic proteinases such as pepsin, cathepsins D and E. In HIV-infected H9 cells, pepstatin A inhibits part of the intracellular processing of the HIV gag protein with no apparent toxicity on HIV-infected cells. [1]

Pepstatin A laos suppresses the differentiation of osteoclasts by blocking ERK signaling and the inhibition of NFATc1 expression. [2]

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    NCM460 cells

  • Concentrations

    10 μM

  • Incubation Time

    24 h

  • Method

    Cells were treated with indicated concentrations of drug.

Selleck's Pepstatin A has been cited by 33 publications

Truncating NFKB1 variants cause combined NLRP3 inflammasome activation and type I interferon signaling and predispose to necrotizing fasciitis [ Cell Rep Med, 2024, 5(4):101503] PubMed: 38593810
Retinoic acid alleviates rotavirus-induced intestinal damage by regulating redox homeostasis and autophagic flux in piglets [ Anim Nutr, 2024, 20:16:409-421.] PubMed: 38371474
Inhibition of PIKfyve Leads to Lysosomal Disorders via Dysregulation of mTOR Signaling [ Cells, 2024, 13(11)953] PubMed: 38891085
Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellular trap phagocytosis [ Sci Rep, 2024, 14(1):16386] PubMed: 39013974
ATF3/SPI1/SLC31A1 Signaling Promotes Cuproptosis Induced by Advanced Glycosylation End Products in Diabetic Myocardial Injury [ Int J Mol Sci, 2023, 24(2)1667] PubMed: 36675183
A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19 [ Biosensors (Basel), 2023, 13(7)682] PubMed: 37504081
A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19 [ Biosensors (Basel), 2023, 13(7)682] PubMed: 37504081
Cysteinyl leukotriene receptor 1 is a potent regulator of the endosomal-lysosomal system in the ARPE-19 retinal pigment epithelial cell line [ Traffic, 2023, 24(4):177-189] PubMed: 36704929
Mannose ameliorates experimental colitis by protecting intestinal barrier integrity [ Nat Commun, 2022, 13(1):4804] PubMed: 35974017
Lysosomal cathepsin D mediates endogenous mucin glycodomain catabolism in mammals [ Proc Natl Acad Sci U S A, 2022, 119(39):e2117105119] PubMed: 36122205

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.