Pembrolizumab (anti-PD-1)

Catalog No.A2005        Batch: A200504

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Technical Data

CAS No. 1374853-91-4
Formulation PBS buffer, pH 7.2
Isotype Human IgG4
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 98.4%
Protein concentration 5.09mg/ml
Endotoxin Level ≤1 EU/mg

Biological Activity

Description Pembrolizumab (anti-PD-1) is a potent, highly selective, fully humanized immunoglobulin (Ig) G4-kappa monoclonal antibody against PD-1 with potential immune checkpoint inhibitory and antineoplastic activities. MW : 146.286 KD.
Targets
PD-1 [1]
(Cell-free assay)
In vitro

The level of LDH and apoptosis are significantly higher in those treated with dexamethasone plus PD1 inhibitor pembrolizumab than other groups.[1]
In vivo

Pembrolizumab and dexamethasone synergistically suppress tumor growth of pancreatic cancer in vivo.[1]

Protocol (Only for Reference)

Cell Assay:

  • Cancer cells were incubated with dexamethasone for 48 h followed by co-cultured with PBMCs with effector/target ratio of 10:1 and the addition of PD1 inhibitor (pembrolizumab; 5 μg/mL) for another 48 h. LDH level was detected.
Animal Study:

  • Animal Models: Immune reconstituted NOD/SCID mice model
    Dosages: 5 mg/kg
    Administration: i.v.
    Reference: [1]
     

Selleck's Pembrolizumab (anti-PD-1) has been cited by 57 publications

Regorafenib plus nivolumab in unresectable hepatocellular carcinoma: the phase 2 RENOBATE trial [ Nat Med, 2024, 10.1038/s41591-024-02824-y] PubMed: 38374347
The BCL-2 inhibitor APG-2575 resets tumor-associated macrophages toward the M1 phenotype, promoting a favorable response to anti-PD-1 therapy via NLRP3 activation [ Cell Mol Immunol, 2024, 21(1):60-79] PubMed: 38062129
Multi-omic profiling reveals the endogenous and neoplastic responses to immunotherapies in cutaneous T cell lymphoma [ Cell Rep Med, 2024, S2666-3791(24)00196-4] PubMed: 38670099
Favorable impact of PD1/PD-L1 antagonists on bone remodeling: an exploratory prospective clinical study and ex vivo validation [ J Immunother Cancer, 2024, 12(5)e008669] PubMed: 38702145
Deprivation of methionine inhibits osteosarcoma growth and metastasis via C1orf112-mediated regulation of mitochondrial functions [ Cell Death Dis, 2024, 15(5):349] PubMed: 38769167
VentX promotes tumor specific immunity and efficacy of immune checkpoint inhibitors [ iScience, 2024, 27(1):108731] PubMed: 38299030
DNA hypo-methylation and expression of GBP4 induces T cell exhaustion in pancreatic cancer [ Cancer Immunol Immunother, 2024, 73(10):208] PubMed: 39110249
ALK5/VEGFR2 dual inhibitor TU2218 alone or in combination with immune checkpoint inhibitors enhances immune-mediated antitumor effects [ Cancer Immunol Immunother, 2024, 73(10):190] PubMed: 39105882
Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors [ Life Sci Alliance, 2024, 7(3)e202302368] PubMed: 38176728
[ BMC Cancer, 2024, ] PubMed: 38409035

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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