Patchouli alcohol

Catalog No.S3778 Batch:S377801

Technical Data

Formula	C₁₅H₂₆O
Molecular Weight	222.37	CAS No.	5986-55-0
Solubility (25°C)*	In vitro	DMSO	44 mg/mL (197.86 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Patchouli alcohol (Patchoulol, Patchouli camphor), an ingredient in pogostemonis herba, has various pharmacological activities and acts as an inhibitor of asexual fungal propagation, inflammation, the influenza virus, and tumorigenesis.
In vitro	Patchouli alcohol inhibits the overexpression of iNOS and IL-6 in protein and mRNA levels in LPS-stimulated RAW264.7 and TNF-a stimulated HT-29 cells. Patchouli alcohol inhibits IkB-α degradation and p65 nuclear translocation, and subsequently suppresses transcriptional activity of NF-kB in LPS-stimulated RAW264.7 and TNF-α-stimulated HT-29 cells. In addition, Patchouli alcohol inhibits LPS- or TNF-α-stimulated ERK1/2 activation by decreasing phosphorylation of ERK1/2^[1].

Description

Patchouli alcohol (Patchoulol, Patchouli camphor), an ingredient in pogostemonis herba, has various pharmacological activities and acts as an inhibitor of asexual fungal propagation, inflammation, the influenza virus, and tumorigenesis.

In vitro

Patchouli alcohol inhibits the overexpression of iNOS and IL-6 in protein and mRNA levels in LPS-stimulated RAW264.7 and TNF-a stimulated HT-29 cells. Patchouli alcohol inhibits IkB-α degradation and p65 nuclear translocation, and subsequently suppresses transcriptional activity of NF-kB in LPS-stimulated RAW264.7 and TNF-α-stimulated HT-29 cells. In addition, Patchouli alcohol inhibits LPS- or TNF-α-stimulated ERK1/2 activation by decreasing phosphorylation of ERK1/2^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines RAW264.7 and HT-29 cells Concentrations 50 and 75 μM Incubation Time 21 h Method RAW264.7 and HT-29 cells (2×10⁵ cells/well) are seeded in 12-well plates and incubated overnight. Then, plasmid mixtures containing 0.5 μg of pNF-kB-Luc plasmid and 0.1 μg of pRL-null vector are transfected for 24 h (for RAW264.7) and 48 h (for HT-29). The empty pGL3-basic vector is used as a negative control. After transfection, cells are pre-treated with Patchouli alcohol, tolfenamic acid (TA), or sulindac sulfide (SS) for 6 h and then co-treated with LPS (for RAW264.7) or TNF-α (for HT-29) for an additional 15 h. The cells are harvested in 1×luciferase lysis buffer, and luciferase activity is measured and normalized to the pRL-null luciferase activity using a dual-luciferase assay kit.

Cell Assay:^{^[1]}

Cell lines
RAW264.7 and HT-29 cells
Concentrations
50 and 75 μM
Incubation Time
21 h
Method
RAW264.7 and HT-29 cells (2×10⁵ cells/well) are seeded in 12-well plates and incubated overnight. Then, plasmid mixtures containing 0.5 μg of pNF-kB-Luc plasmid and 0.1 μg of pRL-null vector are transfected for 24 h (for RAW264.7) and 48 h (for HT-29). The empty pGL3-basic vector is used as a negative control. After transfection, cells are pre-treated with Patchouli alcohol, tolfenamic acid (TA), or sulindac sulfide (SS) for 6 h and then co-treated with LPS (for RAW264.7) or TNF-α (for HT-29) for an additional 15 h. The cells are harvested in 1×luciferase lysis buffer, and luciferase activity is measured and normalized to the pRL-null luciferase activity using a dual-luciferase assay kit.

References

[1] Jeong JB, et al. Food Chem Toxicol. 2013, 55:229-33.

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SHIPPING AND STORAGE
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