Panaxadiol

Catalog No.S3845 Batch:S384501

Technical Data

Formula	C₃₀H₅₂O₃
Molecular Weight	460.73	CAS No.	19666-76-3
Solubility (25°C)*	In vitro	Ethanol	22 mg/mL (47.75 mM)
		DMSO	Insoluble
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Panaxadiol (20(R)-Panaxadiol) is a triterpene sapogenin originally found in species of Panax (ginseng) and exhibits anticancer, cardioprotective, anti-arrhythmic, and antioxidative activities. It inhibits Ca2+ channels, decreasing channel open time and open state probability in vitro and displaying anti-arrhythmic potential.
In vitro	Panaxadiol enhances the anti-cancer eVects of 5-FU on human colorectal cancer cells through the regulation of cell cycle transition and the induction of apoptotic cells^[1]. Panaxadiol inhibits DNA synthesis in a dose-dependent manner with IC50 values ranging from 0.8 to 1.2 µM in SK-HEP-1 cells and HeLa cells. Panaxadiol-treated cells are arrested at G 1/S phase, which coincides well with decreases in Cyclin A-Cdk2 activity, but not in Cyclin E-Cdk2 and Cdc2 activities. The intracellular levels of p21^WAF1/CIP1 are significantly and selectively elevated in a dose- and time-dependent manner in panaxadiol-treated HeLa cells^[2].

Description

Panaxadiol (20(R)-Panaxadiol) is a triterpene sapogenin originally found in species of Panax (ginseng) and exhibits anticancer, cardioprotective, anti-arrhythmic, and antioxidative activities. It inhibits Ca2+ channels, decreasing channel open time and open state probability in vitro and displaying anti-arrhythmic potential.

In vitro

Panaxadiol enhances the anti-cancer eVects of 5-FU on human colorectal cancer cells through the regulation of cell cycle transition and the induction of apoptotic cells^[1]. Panaxadiol inhibits DNA synthesis in a dose-dependent manner with IC50 values ranging from 0.8 to 1.2 µM in SK-HEP-1 cells and HeLa cells. Panaxadiol-treated cells are arrested at G 1/S phase, which coincides well with decreases in Cyclin A-Cdk2 activity, but not in Cyclin E-Cdk2 and Cdc2 activities. The intracellular levels of p21^WAF1/CIP1 are significantly and selectively elevated in a dose- and time-dependent manner in panaxadiol-treated HeLa cells^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HCT-116 Concentrations 5, 15, or 25 μM Incubation Time 24 and 48 h Method HCT-116 cells are incubated with varying concentrations of 5-FU (5, 10, 20, 30, 50, or 100 μM) and panaxadiol (5, 15, or 25 μM) for 24 and 48 h.

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.