Palmatine

Catalog No.S3769 Batch:S376901

Technical Data

Formula	C₂₁H₂₂NO₄
Molecular Weight	352.4	CAS No.	3486-67-7
Solubility (25°C)*	In vitro	DMSO	70 mg/mL (198.63 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Palmatine (Berbericinine, Burasaine), an isoquinoline alkaloid, has sedative, antidepressant, antioxidative, anti-ulcerative, antacid, anticancer, and anti-metastatic activities.

Targets

AChE ^[3] (Cell-free assay)	BChE ^[3] (Cell-free assay)
0.51 μM	6.84 μM

In vitro

Palmatine plays an important role in osteoclast apoptosis via the NOS system in osteoclasts^[1]. Palmatine has been reported to possess sedative and antioxidant activities. It has been also shown to be inhibitor of beta-site amyloid precursor protein-cleaving enzyme 1 (BACE 1), acetyl- and butyrylcholinesterases (AChE and BChE)^[2].

In vivo

Palmatine shows memory enhancing activity in mice probably by inhibiting brain acetylcholinesterase activity, through involvement of GABA-benzodiazepine pathway and due to its antioxidant activity^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines RAW 264.7 cells Concentrations 1, 5, 10, 40, and 100 µM Incubation Time 24 h or 5 days Method Cell viability is measured using the cell counting kit-8 (CCK-8). The cells are seeded in a 96-well flat-bottomed microplate (5 × 10³ cells/well) and cultured in 100 μL of growth medium at 37°C and 5% CO2 for 24 h or 5 days. The cell culture medium in each well is then replaced with 100 μL of cell growth medium containing palmatine at the following concentrations: 1, 5, 10, 40, and 100 µM. Additionally, L-NAME (10, 100, and 1000 μM) dissolved in PBS was added. After incubation for 24 h or 5 days at 37°C, the cells are washed with PBS 3 times. Then, 10 μL of CCK-8 dye and 100 μL of α-MEM cell culture medium are added to each well, and cells are incubated for another 1 h at 37°C. Cell viability (%) is calculated.
Animal Study:^{^[2]}	Animal Models Swiss young male albino mice Dosages 0.1, 0.5, 1 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
RAW 264.7 cells
Concentrations
1, 5, 10, 40, and 100 µM
Incubation Time
24 h or 5 days
Method
Cell viability is measured using the cell counting kit-8 (CCK-8). The cells are seeded in a 96-well flat-bottomed microplate (5 × 10³ cells/well) and cultured in 100 μL of growth medium at 37°C and 5% CO2 for 24 h or 5 days. The cell culture medium in each well is then replaced with 100 μL of cell growth medium containing palmatine at the following concentrations: 1, 5, 10, 40, and 100 µM. Additionally, L-NAME (10, 100, and 1000 μM) dissolved in PBS was added. After incubation for 24 h or 5 days at 37°C, the cells are washed with PBS 3 times. Then, 10 μL of CCK-8 dye and 100 μL of α-MEM cell culture medium are added to each well, and cells are incubated for another 1 h at 37°C. Cell viability (%) is calculated.

Animal Study:^{^[2]}

Animal Models
Swiss young male albino mice
Dosages
0.1, 0.5, 1 mg/kg
Administration
i.p.

References

Selleck's Palmatine has been cited by 1 publication

Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway [ Eur J Pharmacol, 2024, 967:176395]	PubMed: 38350592

Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway [ Eur J Pharmacol, 2024, 967:176395]

PubMed: 38350592

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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