PACAP 1-27

Catalog No.S8414 Batch:S841401

Technical Data

Formula	C₁₄₂H₂₂₄N₄₀O₃₉S
Molecular Weight	3147.61	CAS No.	127317-03-7
Solubility (25°C)*	In vitro	Water	100 mg/mL (31.77 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Pituitary adenylate cyclase activating polypeptide (PACAP 1-27) is a potent PACAP receptor antagonist.

Targets

PACAP receptor ^[1]

In vitro

PACAP27(100 nM) inhibits all Tat-mediated toxic effects including DNA DSBs, and it prevents the induction of neuronal loss induced by Tat(transactivator of transcription). PACAP27, through CCL5, mitigates the effects of Tat-induced neuronal dysfunction exerting its neuroprotective effect^[4]. PACAP-27 (EC50=10nM) stimulates glibenclamide-sensitive and DIDS-insensitive iodide efflux in Calu-3 cells^[5].

In vivo

PACAP-27 is potent VIP-like vasodilators of the femoral arterial bed in dogs. In the cardiovascular system, PACAP27 has vasodepressor actions similar to VIP in anesthetized rats. Intra-arterial infusion of PACAP27 induces dose-related increases in peak femoral arterial flow^[3].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines The pulmonary carcinoma cell line CALU-6 Concentrations 10 pmol/L to 1 μmol/L Incubation Time 20 h Method Cells are incubated with PACAP (1-27) (10 pmol/L to 1 μmol/L) or forskolin (5 μmol/L) for 20 h. Thereafter, cells are harvested, and isolation of total RNA, reverse transcriptase-PCR, and subsequent determination of renin mRNA and β-actin mRNA expression using real-time PCR.
Animal Study:^{^[3]}	Animal Models beagle dogs Dosages 0.25, 0.5, 1, 2.5, 5, 10, and 25 pmol/kg Administration Intra-arterial infusion

Cell Assay:^{^[2]}

Cell lines
The pulmonary carcinoma cell line CALU-6
Concentrations
10 pmol/L to 1 μmol/L
Incubation Time
20 h
Method
Cells are incubated with PACAP (1-27) (10 pmol/L to 1 μmol/L) or forskolin (5 μmol/L) for 20 h. Thereafter, cells are harvested, and isolation of total RNA, reverse transcriptase-PCR, and subsequent determination of renin mRNA and β-actin mRNA expression using real-time PCR.

Animal Study:^{^[3]}

Animal Models
beagle dogs
Dosages
0.25, 0.5, 1, 2.5, 5, 10, and 25 pmol/kg
Administration
Intra-arterial infusion

References

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SHIPPING AND STORAGE
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