Oxyresveratrol

Catalog No.S4739 Batch:S473901

Technical Data

Formula	C₁₄H₁₂O₄
Molecular Weight	244.24	CAS No.	29700-22-9
Solubility (25°C)*	In vitro	DMSO	48 mg/mL (196.52 mM)
		Ethanol	48 mg/mL (196.52 mM)
		Water	1 mg/mL (4.09 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Oxyresveratrol (Tetrahydroxystilbene, 2,3',4,5'-tetrahydroxystilbene), a naturally occurring compound particularly found in Morus alba L., exhibits a potent inhibitory effect on dopa oxidase activity of tyrosinase which catalyzes rate-limiting steps of melanin biosynthesis.

Targets

tyrosinase ^[1]	DPPH ^[2]
	28.9 μM

In vitro

Oxyresveratrol has been reported to inhibit β-amyloid-induced neurotoxicity in cultured cortical neurons and in vitro activity of β-secretase, an enzyme vital for the production of the Aβ protein. And it can also prevent neurotoxicity and DNA damage through its antioxidant properties in cultured neuronal cells. It shows modest inhibitory activity against H5N1 neuraminidase (an enzyme found in avian influenza virus), but strong inhibition against α-glucosidase, an enzyme responsible for the breaking down of starch and polysaccharides into glucose in the intestine^[1]. Oxyresveratrol is an effective radical scavenger^[2]. Oxyresveratrol protects hepatocytes against oxidative stress, mitochondrial damage, and resultant cell death. It induces activation of an antioxidant transcription factor, Nrf2, mediated through ERK phosphorylation^[3].

In vivo

Oxyresveratrol has been reported to undergo extensive hepatic metabolism and rapid urinary elimination, resulting in a short half-life time, and thereby restricting its clinical use. Moreover, its postulated function as a preventive agent for neurodegenerative disorders, such as AD or PD, appears to be hindered by its low ability to cross the blood-brain barrier (BBB). Despite its moderate in vitro inhibitory activity against herpes simplex virus type I (HSV-1), oxyresveratrol, in the form of topical cream, exhibits high therapeutic efficacy in mice cutaneously infected with HSV-1, with a potency comparable to that of the antiviral drug acyclovir^[1]. Oxyresveratrol ameliorates acute liver injury caused by CCl4 in mice^[3].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines N9 microglial cells Concentrations 25, 50, and 100 μM Incubation Time 18 h Method Cytotoxicity assays are performed by the trypan blue method. The cell cultures (N9 or C6 cell line, 2 mL medium each) are treated with 100 μL trypsin-EDTA, incubated for 5 min at 37℃, and then suspended with a pipette. The cell suspension is treated with 20 μL of 0.4% (wt/vol) trypan blue solution and the ratio of living/dead cells is determined immediately by counting the cells in a 20 μL aliquot in a Neubauer chamber under a microscope.
Animal Study:^{^[3]}	Animal Models C57BL/6 male mice Dosages 10 or 30 mg/kg Administration oral administration

Cell Assay:^{^[2]}

Cell lines
N9 microglial cells
Concentrations
25, 50, and 100 μM
Incubation Time
18 h
Method
Cytotoxicity assays are performed by the trypan blue method. The cell cultures (N9 or C6 cell line, 2 mL medium each) are treated with 100 μL trypsin-EDTA, incubated for 5 min at 37℃, and then suspended with a pipette. The cell suspension is treated with 20 μL of 0.4% (wt/vol) trypan blue solution and the ratio of living/dead cells is determined immediately by counting the cells in a 20 μL aliquot in a Neubauer chamber under a microscope.

Animal Study:^{^[3]}

Animal Models
C57BL/6 male mice
Dosages
10 or 30 mg/kg
Administration
oral administration

References

Selleck's Oxyresveratrol has been cited by 1 publication

Main Determinants Affecting the Antiproliferative Activity of Stilbenes and Their Gut Microbiota Metabolites in Colon Cancer Cells: A Structure-Activity Relationship Study [ Int J Mol Sci, 2022, 23(23)15102]	PubMed: 36499424

Main Determinants Affecting the Antiproliferative Activity of Stilbenes and Their Gut Microbiota Metabolites in Colon Cancer Cells: A Structure-Activity Relationship Study [ Int J Mol Sci, 2022, 23(23)15102]

PubMed: 36499424

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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