Oseltamivir Phosphate

Catalog No.S2597 Batch:S259708

Technical Data

Formula	C₁₆H₂₈N₂O₄.H₃PO₄
Molecular Weight	410.4	CAS No.	204255-11-8
Solubility (25°C)*	In vitro	DMSO	82 mg/mL (199.8 mM)
		Water	82 mg/mL (199.8 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Oseltamivir Phosphate(Tamiflu) is a potent and selective inhibitor of the neuraminidase that is essential for replication of influenza A and B viruses, used to prevent influenza.

Targets

Neuraminidase ^[1]

In vitro

Oseltamivir Phosphate, anti-Neu1 antibodies, and matrix metalloproteinase-9-specific inhibitor blocks Neu1 activity associated with EGF-stimulated TNBC MDA-MB-231 cells^[2]. Oseltamivir slows the spread of influenza (flu) virus between cells in the body by stopping the virus from chemically cutting ties with its host cell.

In vivo

Oseltamivir Phosphate monotherapy may be the effective treatment therapy for TNBC(Triple-negative breast cancers)^[2]. It is efficacious in treating infections caused by H5N1 and H9N2 influenza viruses in mice^[3]. After dosing, the prodrug (Oseltamivir phosphate) is readily absorbed from the gastrointestinal tract and rapidly converted into the active metabolite, OC. In all patient groups, OC has high bioavailability and is systemically distributed to infection sites at concentrations sufficient to inhibit a range of influenza virus neuraminidases^[4].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines MDA-MB-231 cells/MCF-7 cells Concentrations 500, 600, 700, 800 μg/mL Incubation Time 24, 48, and 72 h Method Cells are incubated in 96-well plates (5,000 cells/well) and allowed to adhere for 24 hours in 1× DMEM media containing 10% FCS. The media are replaced with fresh DMEM media containing 5% FCS with or without various concentrations of tamoxifen or OP for indicated time periods. Cell viability is tested using WST-1 cell proliferation assay.
Animal Study:^{^[2]}	Animal Models RAGxCγ double mutant mouse Dosages 30 or 50 mg/kg Administration i.p.

Cell Assay:^{^[2]}

Cell lines
MDA-MB-231 cells/MCF-7 cells
Concentrations
500, 600, 700, 800 μg/mL
Incubation Time
24, 48, and 72 h
Method
Cells are incubated in 96-well plates (5,000 cells/well) and allowed to adhere for 24 hours in 1× DMEM media containing 10% FCS. The media are replaced with fresh DMEM media containing 5% FCS with or without various concentrations of tamoxifen or OP for indicated time periods. Cell viability is tested using WST-1 cell proliferation assay.

Animal Study:^{^[2]}

Animal Models
RAGxCγ double mutant mouse
Dosages
30 or 50 mg/kg
Administration
i.p.

References

[4] Davies BE, et al. J Antimicrob Chemother. 2010, 65 Suppl 2:ii5-ii10.

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Customer Product Validation

: , , Infect Immun, 2015, 83(5):2030-42.

Selleck's Oseltamivir Phosphate has been cited by 8 publications

The critical role of RAGE in severe influenza infection: A target for control of inflammatory response in the disease [ Clin Immunol, 2024, 262:110178]	PubMed: 38460892
H3N2 influenza hemagglutination inhibition method qualification with data driven statistical methods for human clinical trials [ Front Immunol, 2023, 14:1155880]	PubMed: 37090729
Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages [ iScience, 2022, 25(4):104088]	PubMed: 35402869
Giardial lipid rafts share virulence factors with secreted vesicles and participate in parasitic infection in mice [ Front Cell Infect Microbiol, 2022, 12:974200]	PubMed: 36081774
Antiviral effect of fufang yinhua jiedu (FFYH) granules against influenza A virus through regulating the inflammatory responses by TLR7/MyD88 signaling pathway [ J Ethnopharmacol, 2021, S0378-8741(21)00290-7]	PubMed: 33813013
Identification of a novel compound targeting the nuclear export of influenza A virus nucleoprotein. [ J Cell Mol Med, 2018, 22(3):1826-1839]	PubMed: 29193684
The Assembly of GM1 Glycolipid and Cholesterol-Enriched Raft-Like Membrane Microdomains is Important for Giardial Encystation. [De Chatterjee A, et al. Infect Immun, 2015, 10.1128/IAI.03118-14]	PubMed: 25733521
Hepatic, intestinal, renal, and plasma hydrolysis of prodrugs in human, cynomolgus monkey, dog, and rat: implications for in vitro-in vivo extrapolation of clearance of prodrugs. [ Drug Metab Dispos, 2014, 42(9):1522-31]	PubMed: 24994071

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.