Oligomycin A

Catalog No.S1478 Batch:S147806

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Technical Data

Formula

C45H74O11
Molecular Weight 791.06 CAS No. 579-13-5
Solubility (25°C)* In vitro DMSO 100 mg/mL (126.41 mM)
Water ˂1 mg/mL
Ethanol ˂1 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.
Targets
ATP synthase [1]
(Cell-free assay)
In vitro

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. [1]

In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. [2]

Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. [3]

Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. [4]
In vivo

Oligomycin A (MCH 32) is an inhibitor of mitochondrial F0F1-ATPase.

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    H1229

  • Concentrations

    100 ng/ml

  • Incubation Time

    1 h

  • Method

    ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
Animal Study:

[5]
  • Animal Models

    Male Sprague-Dawley rats

  • Dosages

    0.5 mg/kg

  • Administration

    i.v.

Customer Product Validation

Data from [Data independently produced by , , Sci Rep, 2016, 6:24641.]

Data from [Data independently produced by , , Int J Oncol, 2018, 53(6):2590-2604]

Data from [Data independently produced by , , PLoS One, 2017, 12(4):e0176355]

Selleck's Oligomycin A has been cited by 113 publications

Mitochondrial-cytochrome c oxidase II promotes glutaminolysis to sustain tumor cell survival upon glucose deprivation [ Nat Commun, 2025, 16(1):212] PubMed: 39747079
Aberrant activation of adenine nucleotide translocase 3 promotes progression and chemoresistance in multiple myeloma dependent on PINK1 transport [ Int J Biol Sci, 2025, 21(1):233-250] PubMed: 39744418
Non-canonical hepatic androgen receptor mediates glucagon sensitivity in female mice through the PGC1α/ERRα/mitochondria axis [ Cell Rep, 2025, 44(1):115188] PubMed: 39792556
PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response [ Elife, 2025, 13RP102852] PubMed: 39878441
Autophagic flux-lipid droplet biogenesis cascade sustains mitochondrial fitness in colorectal cancer cells adapted to acidosis [ Cell Death Discov, 2025, 11(1):21] PubMed: 39856069
TGF-β secreted by cancer cells-platelets interaction activates cancer metastasis potential by inducing metabolic reprogramming and bioenergetic adaptation [ J Cancer, 2025, 16(4):1310-1323] PubMed: 39895802
AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis [ Nat Commun, 2024, 15(1):686] PubMed: 38263319
Spautin-1 promotes PINK1-PRKN-dependent mitophagy and improves associative learning capability in an alzheimer disease animal model [ Autophagy, 2024, 1-22.] PubMed: 39051473
Adenosine diphosphate released from stressed cells triggers mitochondrial transfer to achieve tissue homeostasis [ PLoS Biol, 2024, 22(8):e3002753] PubMed: 39163396
IL-27 disturbs lipid metabolism and restrains mitochondrial activity to inhibit γδ T17 cell-mediated skin inflammation [ Cell Death Dis, 2024, 15(7):491] PubMed: 38982043

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.