Oligomycin A

Catalog No.S1478 Batch:S147806

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Technical Data

Formula

C45H74O11
Molecular Weight 791.06 CAS No. 579-13-5
Solubility (25°C)* In vitro DMSO 100 mg/mL (126.41 mM)
Water ˂1 mg/mL
Ethanol ˂1 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.
Targets
ATP synthase [1]
(Cell-free assay)
In vitro

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. [1]

In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. [2]

Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. [3]

Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. [4]
In vivo

Oligomycin A (MCH 32) is an inhibitor of mitochondrial F0F1-ATPase.

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    H1229

  • Concentrations

    100 ng/ml

  • Incubation Time

    1 h

  • Method

    ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
Animal Study:

[5]
  • Animal Models

    Male Sprague-Dawley rats

  • Dosages

    0.5 mg/kg

  • Administration

    i.v.

Customer Product Validation

Data from [Data independently produced by , , Sci Rep, 2016, 6:24641.]

Data from [Data independently produced by , , Int J Oncol, 2018, 53(6):2590-2604]

Data from [Data independently produced by , , PLoS One, 2017, 12(4):e0176355]

Selleck's Oligomycin A has been cited by 104 publications

AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis [ Nat Commun, 2024, 15(1):686] PubMed: 38263319
Spautin-1 promotes PINK1-PRKN-dependent mitophagy and improves associative learning capability in an alzheimer disease animal model [ Autophagy, 2024, 1-22.] PubMed: 39051473
Adenosine diphosphate released from stressed cells triggers mitochondrial transfer to achieve tissue homeostasis [ PLoS Biol, 2024, 22(8):e3002753] PubMed: 39163396
IL-27 disturbs lipid metabolism and restrains mitochondrial activity to inhibit γδ T17 cell-mediated skin inflammation [ Cell Death Dis, 2024, 15(7):491] PubMed: 38982043
Multivariate analysis of metabolic state vulnerabilities across diverse cancer contexts reveals synthetically lethal associations [ Cell Rep, 2024, 43(10):114775] PubMed: 39305483
Macrophage NRF1 promotes mitochondrial protein turnover via the ubiquitin proteasome system to limit mitochondrial stress and inflammation [ Cell Rep, 2024, 43(10):114780] PubMed: 39325625
Hypoxia promotes metastasis by relieving miR-598-3p-restricted glycolysis in gastric cancer [ J Transl Med, 2024, 22(1):283] PubMed: 38491378
SFRP2 modulates functional phenotype transition and energy metabolism of macrophages during diabetic wound healing [ Front Immunol, 2024, 15:1432402] PubMed: 39464880
Novel therapeutic strategies targeting bypass pathways and mitochondrial dysfunction to combat resistance to RET inhibitors in NSCLC [ Biochim Biophys Acta Mol Basis Dis, 2024, 1870(6):167249] PubMed: 38768929
Role of oxidative phosphorylation in the antidepressant effects of arketamine via the vagus nerve-dependent spleen-brain axis [ Neurobiol Dis, 2024, 199:106573] PubMed: 38901783

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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