Oleuropein

Catalog No.S7867 Batch:S786705

Technical Data

Formula

C₂₅H₃₂O₁₃

Molecular Weight

540.51

CAS No.

32619-42-4

Solubility (25°C)*

In vitro

DMSO

250 mg/mL (462.52 mM)

Ethanol

100 mg/mL (185.01 mM)

Water

63 mg/mL (116.55 mM)

In vivo (Add solvents to the product individually and in order)

Clear solution

PBS

30.0mg/ml

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Oleuropein is an antioxidant polyphenol isolated from olive leaf. Oleuropein exerts antioxidant, anti-inflammatory and anti-atherogenic effects and suppresses the adipocyte differentiation in vitro. Oleuropein inhibits PPARγ activity. Oleuropein exerts anti-adipogenic effect through direct inhibition of PPARγ transcriptional activity. Oleuropein also inhibits aromatase. Oleuropein induces apoptosis. Phase 2.
In vitro	In both J774A.1 cells and primary mouse macrophages, Oleuropein increases LPS-induced NO production. ^[1] Oleuropein prevents oxidative myocardial injury induced by ischemia and reperfusion. ^[2] In TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib cells, Oleuropein inhibits cell proliferation and migration. ^[3]
In vivo	In swiss albino mice that spontaneously develop soft tissue sarcomas, Oleuropein (i.p. or p.o.) completely regresses tumors. ^[3] In rats, Oleuropein (40 mg/kg, p.o.) protects from cardiac remodeling process after isoproterenol-induced myocardial infarction through inhibiting angiotensin-converting enzyme activity. ^[4]

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib cells Concentrations -- Incubation Time 5 days Method Approximately 5 × 10³ cells (TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib) are seeded in triplicate wells of 96-well plates in 100 μl of their respective growth medium with increasing doses of purified Oleuropein. Cell proliferation is determined on day 5 using the CellTiter MTS cell proliferation kit according to the manufacturer’s instructions. Absorbance at 490 nm is measured with a Bio-Tek Instruments microplate reader. Background readings are subtracted from the experimental readings.
Animal Study:^{^[3]}	Animal Models Swiss albino mice that spontaneously develops soft tissue sarcomas Dosages 50 μg/mouse Administration i.p. or p.o.

Cell Assay:^{^[3]}

Cell lines
TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib cells
Concentrations
--
Incubation Time
5 days
Method
Approximately 5 × 10³ cells (TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib) are seeded in triplicate wells of 96-well plates in 100 μl of their respective growth medium with increasing doses of purified Oleuropein. Cell proliferation is determined on day 5 using the CellTiter MTS cell proliferation kit according to the manufacturer’s instructions. Absorbance at 490 nm is measured with a Bio-Tek Instruments microplate reader. Background readings are subtracted from the experimental readings.

Animal Study:^{^[3]}

Animal Models
Swiss albino mice that spontaneously develops soft tissue sarcomas
Dosages
50 μg/mouse
Administration
i.p. or p.o.

References

[4] Mnafgui K, et al. Toxicol Mech Methods. 2015, 1-9.

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Selleck's Oleuropein has been cited by 2 publications

Transcriptome profiling reveals superovulation with the gonadotropin-releasing hormone agonist trigger impaired embryo implantation in mice [ Front Endocrinol (Lausanne), 2024, 15:1354435]	PubMed: 38469140
An antiviral drug screening system for enterovirus 71 based on an improved plaque assay: A potential high-throughput method [ J Med Virol, 2019, 91(8):1440-1447]	PubMed: 30900754

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.